Effect of glutamate receptor antagonists and antirheumatic drugs on proliferation of synoviocytes in vitro

European Journal of Pharmacology
Jolanta Parada-TurskaWaldemar A Turski

Abstract

One of the most striking features of inflammatory arthritis is the hyperplasia of synovial fibroblasts. It is not known whether the massive synovial hyperplasia characteristic of rheumatoid arthritis is due to the proliferation of synovial fibroblasts or to defective apoptosis. It has been found that glutamate receptor antagonists inhibit proliferation of different human tumour cells and the anticancer potential of glutamate receptor antagonists was suggested. Here, we investigated the effect of glutamate receptor antagonists and selected antirheumatic drugs on proliferation of synoviocytes in vitro. Experiments were conducted on rabbit synoviocytes cell line HIG-82 obtained from American Type Culture Collection (Menassas, VA, USA). Cell proliferation was assessed by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The IC50 value (the concentration of drug necessary to induce 50% inhibition) together with confidence limits was calculated. Glutamate receptor antagonists, 1-(4-aminophenyl)-3,5-dihydro-7,8-dimethoxy-4H-2,3-benzodiazepin-4-one (CFM-2), riluzole, memantine, 1-4-aminophenyl-methyl-7,8-methylenedioxy-5H-2,3-benzodiazepine (GYKI 52466), dizocilpine, ketamine and 2,3-dihydroxy-6-nitro-7...Continue Reading

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Citations

Sep 23, 2011·Annals of Biomedical Engineering·Tatsuya NakamuraEiji Tanaka
Dec 17, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Terry A McNearneyKarin N Westlund
Oct 1, 2011·Arthritis Research & Therapy·Manon C ZweersSimon C Mastbergen
Mar 7, 2012·Medical Hypotheses·Dennis J McCarthyGerard Sanacora
Jun 5, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Benjamin John Floyd DeanAndrew Jonathan Carr
Jul 19, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Laurent BédouetAlexandre Laurent

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