Effect of glutathione depletion on Leydig cell steroidogenesis in young and old brown Norway rats.

Endocrinology
Haolin ChenB R Zirkin

Abstract

Changes in the oxidant/antioxidant environment of aging Leydig cells have been shown to be correlated with the reduced ability of these cells to produce testosterone. With this in mind, we hypothesized that the experimental depletion of glutathione (GSH), an abundant Leydig cell intracellular antioxidant, might result in reduced testosterone production. Incubation of Leydig cells isolated from the testes of adult Brown Norway rats with buthionine sulfoximine (BSO) reduced GSH content by more than 70% and testosterone production by about 40%. The antioxidants vitamin E, N-tert-butyl-alpha-phenylnitrone and Trolox countered BSO's effect on steroidogenesis but not on GSH depletion. Together, BSO and glutathione ethyl ester maintained intracellular GSH and also testosterone production, whereas 1,2-dithiole-3-thione, which increases intracellular GSH, increased testosterone production. In vivo studies also were conducted. Young (4 month old) and old (24 month old) rats were injected with BSO twice a day for 7 d, after which Leydig cells were isolated and analyzed in vitro. BSO treatment reduced Leydig cell GSH content by 70% and the ability of the Leydig cells to produce testosterone by more than 50%. As with aging, decreases were s...Continue Reading

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Citations

May 2, 2014·Journal of Molecular Endocrinology·Syed Kashif ZaidiSalman Azhar
Dec 25, 2012·Toxicology and Applied Pharmacology·Mohammad Amin RezvanfarMohammad Abdollahi
Jan 23, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Gauthier SchangBarbara F Hales
Aug 10, 2015·Nutrition·Michael J GladeMichael M Meguid
Feb 24, 2015·Experimental Gerontology·M C BeattieB R Zirkin
Nov 23, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hamdy A A Aly, Rasha M Khafagy
Jul 28, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Yiyan WangHaolin Chen
Aug 15, 2019·Open Medicine·Łukasz DobrekMarta Kopańska
Apr 10, 2020·Andrologia·Saradha BaskaranRalf Henkel
Jul 20, 2019·Human & Experimental Toxicology·H Aa Aly
Jul 7, 2021·Molecular and Cellular Endocrinology·Peng LuoChunhua Deng

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