Effect of glycation focusing on the process of epidermal lipid synthesis in a reconstructed skin model and membrane fluidity of stratum corneum lipids

Dermato-endocrinology
Mami YokotaYoshihiro Tokudome

Abstract

We previously reported that epidermal glycation causes an increase in saturated fatty acid (FA) content in a differentiated reconstructed skin model and HaCaT cells. However, the relationship between ceramides (CERs) and glycation and their effects on stratum corneum (SC) barrier function was not elucidated. In this study, we investigated the effect of glycation on lipid content in 6-day-old cultured reconstructed skin. We used the EPISKIN RHE 6D model and induced glycation using glyoxal. In addition to transepidermal water loss, content of CERs, cholesterol and FA in the reconstructed epidermal model were analyzed by high performance thin layer chromatography. Expression of genes related to ceramide metabolism was determined by real time RT-PCR. Membrane fluidity of stratum corneum lipid liposomes (SCLL) that mimic glycated epidermis was analyzed using an electron spin resonance technique. It was found that FA was significantly increased by glycation. CER[NS], [AP], and cholesterol were decreased in glycated epidermis. Expression of ceramide synthase 3 (CERS3) was significantly decreased while fatty acid elongase 3 was increased by glyoxal in a dose dependent manner. Membrane fluidity of SCLL mimicking the lipid composition of...Continue Reading

References

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Aug 23, 2016·Skin Pharmacology and Physiology·Mami Yokota, Yoshihiro Tokudome

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Citations

Jan 5, 2021·Biological & Pharmaceutical Bulletin·Chihiro NaitoAkira Yamamoto
May 21, 2021·Advanced Drug Delivery Reviews·Joke A BouwstraAbdoelwaheb El Ghalbzouri

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Methods Mentioned

BETA
PCR
electron spin resonance
ESR

Software Mentioned

[UNK] XRS +
EPISKIN
SAS
Image [UNK]

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