PMID: 6540462Aug 1, 1984Paper

Effect of glycerol and low pH on heat-induced cell killing and loss of cellular DNA polymerase activities in Chinese hamster ovary cells

Radiation Research
N F Mivechi, W C Dewey

Abstract

A whole-cell assay technique for DNA polymerase alpha and beta was used to measure the activities of both enzymes in Chinese hamster ovary (CHO) cells after hyperthermic treatment of 42.2 - 45.5 degrees C in acidic or basic environment and in the presence or absence of 5% glycerol. Cell survival was measured at the same time, and the DNA polymerase activities were correlated with survival. The results show a positive correlation between cell killing by heat and loss of DNA polymerase beta activity, both when cells were sensitized to heat by treatment at pH 6.7 with or without glycerol and when cells were protected from heat by treatment with 5% glycerol at pH 7.4 or 6.7. The results show a poor correlation between loss of DNA polymerase alpha activity and cell survival; i.e., compared to cell killing, the loss of DNA polymerase alpha activity was sensitized to heat more by acidic treatment without glycerol and was protected less from heat by glycerol treatment at normal physiological pH (pH 7.4). However, cell killing and loss of polymerase alpha activity did correlate well for sensitization to heat by acidic treatment in the presence of glycerol and for protection from heat by glycerol treatment at low pH. These results consid...Continue Reading

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