Effect of glycine on Helicobacter pylori in vitro

Antimicrobial Agents and Chemotherapy
Masaaki MinamiMichio Ohta

Abstract

Glycine is the simplest amino acid and is used as a metabolic product in some bacteria. However, an excess of glycine inhibits the growth of many bacteria, and it is used as a nonspecific antiseptic agent due to its low level of toxicity in animals. The effect of glycine on Helicobacter pylori is not precisely known. The present study was conducted to investigate (i) the effect of glycine on clarithromycin (CLR)-resistant and -susceptible strains of H. pylori, (ii) the effect of glycine in combination with amoxicillin (AMX), and (iii) the postantibiotic effect (PAE). The MIC at which 90% of strains are inhibited for glycine was almost 2.5 mg/ml for 31 strains of H. pylori, including CLR-resistant strains. We constructed isogenic CLR-resistant mutant strains by natural transformation and investigated the difference between clinical wild-type strains and isogenic mutants. There were no differences in the MICs between CLR-resistant and -susceptible strains or between clinical wild-type and mutant strains. The combination of AMX and glycine showed synergistic activity, with the minimum bactericidal concentration of AMX with glycine decreasing to 1/10 that of AMX alone. Glycine showed no PAE against H. pylori. These results suggest ...Continue Reading

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Citations

May 26, 2012·Clinical Oral Implants Research·Andrea CochisLia Rimondini
Dec 24, 2005·Annals of Clinical Microbiology and Antimicrobials·Shaik Mahaboob AliNiyaz Ahmed
Dec 20, 2005·Journal of Ethnopharmacology·Byeoung-Soo ParkJeong-Han Kim
Apr 15, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Giuseppina MandalariMichele Navarra
Mar 29, 2020·Journal of Biomolecular Structure & Dynamics·Sefa CelikBelgi Diren Sigirci
Sep 7, 2017·Bioconjugate Chemistry·Amlan ChakrabortyMagdalena Plebanski

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