Effect of graft reperfusion on intracellular calcium levels in mononuclear leucocytes during human orthotopic liver transplantation

The British Journal of Surgery
S M EnrightM C Bellamy

Abstract

Orthotopic liver transplantation (OLT) is accompanied by local and systemic manifestations of the ischaemia-reperfusion syndrome. Local effects are mediated in part through changes in intracellular calcium levels in Kupffer cells. Arachidonic acid metabolites mediate increases in intracellular calcium concentration and thus potentiate the effect of free radicals. This study was carried out to characterize white blood cell (WBC) calcium changes as a mediator for white cell activation in human OLT. Twenty consecutive patients had OLT using standard surgery and anaesthesia techniques. Blood samples were drawn for estimation of WBC cytosolic calcium content at induction of anaesthesia, 5 min before graft reperfusion and 15 min after reperfusion. The rate of rise in intracellular calcium concentration after the addition of a calcium chloride 1 mmol L(-1) solution to the extracellular milieu was used as an estimate of membrane calcium permeability. Both extracellular (P = 0.0002) and intracellular (P = 0.0008) calcium concentrations rose with time. However, at no time was there a correlation between extracellular and intracellular calcium levels or rate of calcium influx (r2 = 0.002, P = 0.78). There was a significant increase in int...Continue Reading

References

Jan 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J C Caldwell-KenkelJ J Lemasters
Jan 1, 1986·American Journal of Surgery·J W WilliamsJ D Massie
Feb 1, 1987·Circulation·E D Wigle
Feb 20, 1967·JAMA : the Journal of the American Medical Association·G E MooreH A Franklin
Oct 1, 1984·Annals of Surgery·B W ShawT E Starzl
Apr 1, 1994·British Journal of Anaesthesia·N R WebsterS A Sadek
Aug 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Y IimuroR G Thurman

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Citations

May 18, 2012·Seminars in Cardiothoracic and Vascular Anesthesia·Matthew FiegelNathaen S Weitzel
Dec 4, 2015·Korean journal of anesthesiology·Sung-Moon Jeong
Feb 6, 2004·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Mark C BellamyUNKNOWN Leeds Liver Group

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