Effect of granulocyte-colony stimulating factor on generation of oxygen-derived free radicals and myeloperoxidase activity in neutrophils from poorly controlled NIDDM patients

Diabetes
N SatoM Mori

Abstract

To evaluate whether granulocyte-colony stimulating factor (G-CSF) improves an impaired production of oxygen-derived free radicals by neutrophils from poorly controlled NIDDM patients, we studied the effect of G-CSF on myeloperoxidase (MPO) activity and chemiluminescence amplified by a Cypridina luciferin analog (CLA-DCL), which is dependent on O2 generation, and luminol (L-DCL), which is dependent on OCl(-) generation, in response to formyl-methonyl-leucyl-phenylalanine. Both CLA-DCL and L-DCL by neutrophils from the diabetic group (n = 15, HbA(1c) >10%) were significantly decreased (26 and 37%, respectively: P < 0.01) compared with the age-matched normal control group (n = 15), and L-DCL was more sensitive to this inhibition than CLA-DCL (P < 0.05). In both control and diabetic neutrophils, G-CSF significantly enhanced both CLA-DCL (175% in control and 156% in diabetic) and L-DCL (283% in control and 346% in diabetic). In diabetic neutrophils, the enhancing effect of G-CSF on L-DCL was more sensitive than on CLA-DCL (P < 0.001). There was a positive correlation between HbA(1c) and the enhancing effect of G-CSF on L-DCL in diabetic patients (P < 0.05), but not on CLA-DCL. MPO activity was also decreased in the diabetic group (6...Continue Reading

Citations

Aug 2, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·G C K W KohW J Wiersinga
Nov 13, 2001·Diabetes, Obesity & Metabolism·A YönemA Corakçi
Jan 11, 2001·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·A GerberH U Schulz
Jul 23, 2009·Antioxidants & Redox Signaling·Betty S van der VeenPeter Heeringa
Mar 26, 2013·Antioxidants & Redox Signaling·Susana Rovira-LlopisVictor M Victor
Jun 12, 2013·Pharmacogenomics·Maria Lucia Corrêa-Giannella, Ubiratan F Machado
Nov 25, 2003·Kidney International·Ernst MalleHermann-Josef Grone
Sep 11, 2007·Infectious Disease Clinics of North America·Smita GuptaNancy Khardori
Sep 9, 2006·Diabetes/metabolism Research and Reviews·Anton Y PelegTimothy M E Davis
Apr 17, 2016·Archives of Dermatological Research·Hannah TrøstrupMagnus S Ågren
Jul 25, 2008·Journal of Clinical Periodontology·Daniela GonçalvesSilvana Regina Perez Orrico
Aug 21, 2013·The Cochrane Database of Systematic Reviews·Mario CrucianiFausto de Lalla
Oct 8, 1999·The Journal of Antimicrobial Chemotherapy·K SatoM Mori
Jul 9, 2014·Proceedings of the National Academy of Sciences of the United States of America·Cristina I Silvescu, Robert Sackstein
Aug 14, 1998·Free Radical Research·H NakaharaK Utsumi
Apr 15, 2011·Metabolism: Clinical and Experimental·Isabelle LhommeauThierry Patrice

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