PMID: 9529068Apr 7, 1998Paper

Effect of group A streptococcal cysteine protease on invasion of epithelial cells

Infection and Immunity
P J TsaiJ J Wu

Abstract

Cysteine protease of group A streptococci (GAS) is considered an important virulence factor. However, its role in invasiveness of GAS has not been investigated. We demonstrated in this study that two strains of protease-producing GAS had the ability to invade A-549 human respiratory epithelial cells. Isogenic protease mutants were constructed by using integrational plasmids to disrupt the speB gene and confirmed by Southern hybridization and Western immunoblot analyses. No extracellular protease activity was produced by the mutants. The mutants had growth rates similar to those of the wild-type strains and produced normal levels of other extracellular proteins. When invading A-549 cells, the mutants had a two- to threefold decrease in activity compared to that of the wild-type strains. The invasion activity increased when the A-549 cells were incubated with purified cysteine protease and the mutant. However, blockage of the cysteine protease with a specific cysteine protease inhibitor, E-64, decreased the invasion activity of GAS. Intracellular growth of GAS was not found in A-549 cells. The presence or absence of protease activity did not affect the adhesive ability of GAS. These results suggested that streptococcal cysteine p...Continue Reading

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Citations

Apr 23, 2005·Infection and Immunity·Chih-Hung WangJiunn-Jong Wu
Feb 25, 2009·The Journal of Biological Chemistry·Chih-Chieh WangWoei-Jer Chuang
Oct 13, 2001·Infection and Immunity·M Collin, A Olsén
Nov 20, 2002·Infection and Immunity·Mattias CollinArne Olsén
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Jan 13, 2006·PLoS Medicine·Pontus ThulinAnna Norrby-Teglund
Jan 18, 2014·Journal of Molecular Recognition : JMR·Raja Mohmed Beema ShafreenShunmugiah Karutha Pandian
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Mar 7, 2003·The Journal of Biological Chemistry·Chiu-Yueh ChenWoei-Jer Chuang
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Oct 16, 2002·Infection and Immunity·Vinod PandiripallyDavid Cue
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Jul 27, 2017·International Wound Journal·Sharon LindsayKatie Bourdillon

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