Effect of growth conditions and staining procedure upon the subsurface transport and attachment behaviors of a groundwater protist.

Applied and Environmental Microbiology
Ronald W HarveyFranco Novarino

Abstract

The transport and attachment behaviors of Spumella guttula (Kent), a nanoflagellate (protist) found in contaminated and uncontaminated aquifer sediments in Cape Cod, Mass., were assessed in flowthrough and static columns and in a field injection-and-recovery transport experiment involving an array of multilevel samplers. Transport of S. guttula harvested from low-nutrient (10 mg of dissolved organic carbon per liter), slightly acidic, granular (porous) growth media was compared to earlier observations involving nanoflagellates grown in a traditional high-nutrient liquid broth. In contrast to the highly retarded (retardation factor of approximately 3) subsurface transport previously reported for S. guttula, the peak concentration of porous-medium-grown S. guttula traveled concomitantly with that of a conservative (bromide) tracer. About one-third of the porous-medium-grown nanoflagellates added to the aquifer were transported at least 2.8 m downgradient, compared to only approximately 2% of the broth-grown nanoflagellates. Flowthrough column studies revealed that a vital (hydroethidine [HE]) staining procedure resulted in considerably less attachment (more transport) of S. guttula in aquifer sediments than did a staining-and-fix...Continue Reading

References

Jul 1, 1997·FEMS Microbiology Reviews·N E KinnerM Kazmierkiewicz-Tabaka
Jul 1, 1997·FEMS Microbiology Reviews·G NovarinoB Teltsch
Nov 5, 1997·Applied and Environmental Microbiology·J Chen, B Koopman
Feb 17, 1998·Applied and Environmental Microbiology·N E KinnerL D Meeker
Feb 1, 1993·Applied and Environmental Microbiology·J L SinclairJ T Wilson
Jan 1, 1995·Applied and Environmental Microbiology·R W HarveyD Metge

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