Effect of growth conditions on yield and heme content of Vitreoscilla.

Journal of Bacteriology
P Lamba, D A Webster

Abstract

Vitreoscilla, a gliding bacterium in the Beggiatoaceae, is an obligate aerobe in which cytochrome o functions as the terminal oxidase. Protoheme IX is the only heme type present in this organism. The yield and heme content of Vitreoscilla cells grown in yeast extract, peptone, and acetate were dependent on growth conditions. Cells harvested in early stationary phase contained roughly three times as much heme as cells in early log phase. There was an optimal shaking rate for maximum heme content of cells harvested in stationary phase at fixed initial nutrient concentration. The heme content of cells grown at a fixed shaking rate increased from 5 nmol/g (wet weight) in media which had low nutrient concentration to a maximum of 45 nmol/g (wet weight) in media which had high nutrient concentration, and there was a corresponding sixfold increase in cytochrome o content and an eightfold increase in respiratory rate, evidence that some of the additional heme was incorporated into respiratory pigments. Heme content may be controlled jointly by competition for oxygen and availability of nutrients. Temperature and initial pH affected the growth rate but not the final yield or heme content. Growth rate was optimal at pH 8.0 to 8.5. A defi...Continue Reading

References

Dec 23, 1977·Science·M D Maines, A Kappas
Dec 1, 1972·Journal of Bacteriology·D C Mayfield, A S Kester
Apr 1, 1966·Plant Physiology·D A Webster, D P Hackett
Jan 1, 1958·Archiv für Mikrobiologie·E R LEADBETTER, J W FOSTER
Jun 1, 1961·Canadian Journal of Microbiology·J W COSTERTONC F ROBINOW
Feb 1, 1951·Journal of General Microbiology·E G PRINGSHEIM

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Citations

Feb 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D E Goldberg
May 15, 1990·Archives of Biochemistry and Biophysics·J J Abrams, D A Webster
Aug 22, 2006·Biochemical and Biophysical Research Communications·Yeon T ChungDale A Webster
Nov 26, 2014·Journal of Agricultural and Food Chemistry·Yung Lee SuenFeng Chen

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