Effect of growth factors on proliferation and expression of growth factor receptors in a human lens epithelial cell line

Journal of Cataract and Refractive Surgery
Juergen KampmeierGabriele E Lang

Abstract

To investigate the effect of basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), insulin-like growth factor 1 (IGF-1), and transforming growth factor beta 2 (TGFbeta2) on proliferation of a human lens epithelial cell line (HLEC-SRA 01/04); the effect of bFGF and TGFbeta2 on proliferation of human lens epithelial cells (HLECs); and the expression of bFGF, EGF, IGF-1, and TGFbeta2 receptors in an HLEC-SRA 01/04 cell line. Department of Ophthalmology, University of Ulm, Ulm, Germany. Both HLEC and HLEC-SRA 01/04 were treated with 1 to 50 ng/mL bFGF and TGFbeta2) Additionally, HLEC-SRA 01/04 were cultured with EGF and IGF-1 at a concentration of 1 to 50 ng/mL for 48 hours in the presence of [3H]-thymidine. In all experiments, untreated serum-free negative controls were used. (3H)-thymidine incorporation as a direct measure of lens epithelial cell proliferation was assessed by liquid scintillation counting. The expression of bFGF, EGF, IGF-1, and TGFbeta2 receptors in HLEC-SRA 01/04 were analyzed by reverse transcriptase polymerase chain reaction (RT-PCR). Statistical analysis was performed using the 2-sample t test for the means. Proliferation of HLECs was dose dependently induced by bFGF and TGFbeta2, showing max...Continue Reading

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Citations

Jan 19, 2013·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·C WertheimerK H Eibl-Lindner
Nov 8, 2011·Ophthalmic Research·Zisis GatzioufasBerthold Seitz
Nov 14, 2013·BMC Ophthalmology·Noriyasu HashidaKohji Nishida
May 6, 2015·Experimental Eye Research·Ian Michael Wormstone, Julie Ann Eldred
Mar 8, 2014·International Journal of Experimental Pathology·Sirilaksana KunjaraPatricia McLean
Dec 5, 2014·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Christian WertheimerKirsten Eibl-Lindner

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