Abstract
Guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S), a specific activator of G proteins, did not change the Kd nor total binding of [125I]insulin in plasma membranes from rat liver. Insulin did not alter GTP gamma 35S binding nor polypeptide ADP ribosylation in crude and plasma membranes catalyzed either intrinsically or by cholera toxin. In L6 muscle cells, insulin caused tyrosine phosphorylation of a polypeptide of Mr 160,000. Cell electroporation enabled testing of G protein action in this cellular system. Phosphorylation of the Mr 160,000 polypeptide in these permeabilized cells was insulin and ATP dependent but other small molecules or ionic gradients were not essential. The reaction could not be mimicked by the G protein agonist GTP gamma S nor inhibited by the G protein antagonist guanosine 5'-O-(2-thiodiphosphate) (GDP beta S). However, GTP gamma S effectively decreased insulin-mediated phosphorylation of this polypeptide. This suggests that the tyrosine kinase activity of the insulin receptor can be modulated by G protein agonists. It is concluded that cross talk between the insulin receptor and G proteins could not be demonstrated in isolated membranes by strategies that detect interactions between beta-adrenergic recep...Continue Reading
References
Nov 14, 1985·Nature·M F WhiteC R Kahn
Jan 1, 1989·Endocrinology·C HofmannJ Whittaker
Jul 1, 1988·Endocrinology·A KlipU M Koivisto
Jul 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J N FainR J Wojcikiewicz
Jan 1, 1985·Methods in Enzymology·F A Ribeiro-NetoR D Sekura
Feb 1, 1989·The Biochemical Journal·P E NasmithS Grinstein
Feb 15, 1987·Archives of Biochemistry and Biophysics·E BurdettA Klip
Feb 6, 1985·Nature·A UllrichM Tsubokawa
May 25, 1987·FEBS Letters·D Gawler, M D Houslay
Sep 30, 1988·Biochemical and Biophysical Research Communications·K MomomuraM Kasuga
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·Y ZickA M Spiegel
Jun 15, 1987·FEBS Letters·R M O'BrienA Hall
Oct 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E R WeissG L Johnson
Feb 15, 1987·The Biochemical Journal·A Klip, T Ramlal
Feb 4, 1988·Nature·S AvissarR H Belmaker
Mar 15, 1988·Biochemical and Biophysical Research Communications·C K HuangJ E Casnellie
Mar 15, 1986·The Biochemical Journal·D E Knight, M C Scrutton
Jun 15, 1985·The Biochemical Journal·C M HeyworthM D Houslay
Aug 1, 1985·Canadian Journal of Physiology and Pharmacology·H J GorenM D Hollenberg
Apr 1, 1969·The Journal of Cell Biology·C S SongA Kappas
Aug 15, 1970·Nature·U K Laemmli
Nov 6, 1984·Biochemistry·T AsanoE M Ross
Oct 31, 1983·Biochemical and Biophysical Research Communications·M L ElksM Vaughan
Jan 1, 1984·Analytical Biochemistry·M S BlakeE C Gotschlich
Citations
Jun 15, 1994·Molecular and Cellular Biochemistry·C BallardS Schaffer
Oct 21, 2003·Autonomic & Autacoid Pharmacology·M R CorbozJ A Hey
Mar 1, 1994·Journal of Cellular Biochemistry·J F CaroJ K Thakkar
Oct 14, 2010·Experimental Lung Research·Michel R CorbozStephen P Eckel
Mar 1, 1995·Endocrine·J L Larsen, T W Burkman
Nov 1, 1993·Journal of Cellular Biochemistry·R Ravindra, J F Caro
Apr 23, 1999·Journal of Basic and Clinical Physiology and Pharmacology·M A Rizzo, G Romero
Jul 29, 1991·FEBS Letters·S PetersenJ Eckel
Jan 1, 1992·Cellular Signalling·R C HonnorC Londos
Sep 1, 1993·Cellular Signalling·A KlipY Mitsumoto
Jun 8, 1993·Biochimica Et Biophysica Acta·S Orlowski, L M Mir
Aug 31, 1990·Biochemical and Biophysical Research Communications·H W Davis, J M McDonald
Feb 15, 1991·Archives of Biochemistry and Biophysics·H ShibataT Kono