Effect of H2O2 on fiber initiation using fiber retardation initiation mutants in cotton (Gossypium hirsutum)

Journal of Plant Physiology
Dayong ZhangWangzhen Guo

Abstract

Single-celled fibers initiate at anthesis from cotton seed epidermal cells of normal developmental cotton cultivars; however, fiber initiation is retarded in some cotton fiber mutants. In this study, the relationship between genes associated with fiber initiation retardation and fiber initiation development was investigated using three cotton fiber developmental mutants: recessive naked seed n2; dominant naked seed N1; and Xinxiangxiaoji Linted-Fuzzless Mutant (XinFLM); with genetic standard line TM-1 (TM-1) as control. Retardation during fiber initiation development was observed in N1 and XinFLM by scanning electron microscope (SEM) analysis. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of genes related to the fiber initiation development showed that the expression of GhEXP1 and GhMYB25 was lower in N1 and XinFLM than in TM-1 and n2, however, the expression of GhTTG1 and GhTTG3 in XinFLM and n2 was higher than in TM-1 and N1. In vivo and in vitro treatments on ovules demonstrated that 30% hydrogen peroxide (H2O2) could prevent fiber initiation retardation in XinFLM, but no evident effect on N1. To further confirm the relationship between gene expression and the effects of H2O2 in XinFLM, qRT-PCR analysis o...Continue Reading

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Citations

Dec 27, 2015·Plant Science : an International Journal of Experimental Plant Biology·Amandeep MittalChristopher D Rock
Dec 3, 2011·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Juan LuoXue-Bao Li
Oct 17, 2017·The New Phytologist·Huaitong WuTianzhen Zhang
Jun 29, 2018·The Plant Journal : for Cell and Molecular Biology·Verandra KumarSamir V Sawant
Mar 27, 2012·The Plant Journal : for Cell and Molecular Biology·Sally-Ann WalfordElizabeth S Dennis
Aug 29, 2020·The New Phytologist·Lu WangYong-Ling Ruan
Jul 18, 2020·International Journal of Biological Macromolecules·Lichen WangMuhammad Mujtaba

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