Effect of health foods on cytochrome P450-mediated drug metabolism

Journal of Pharmaceutical Health Care and Sciences
Takamitsu SasakiKiyoshi Nagata

Abstract

Health foods have been widely sold and consumed in Japan. There has been an increase in reports of adverse effects in association with the expanding health food market. While health food-drug interactions are a particular concern from the viewpoint of safe and effective use of health foods, information regarding such interactions is limited owing to the lack of established methods to assess the effects of health food products on drug metabolism. We therefore developed cells that mimicked the activities of cytochrome P450 1A2 (CYP1A2), CYP2C9, CYP2C19, CYP2D6, and CYP3A4, which strongly contribute to drug metabolism in human hepatocytes, and established a system to assess the inhibitory activity of health foods toward P450-mediated metabolism. We simultaneously infected HepG2 cells with five P450-expressing adenoviruses (Ad-CYP1A2, Ad-CYP2C9, Ad-CYP2C19, Ad-CYP2D6, and Ad-CYP3A4) to mimic the activity levels of these P450s in human hepatocytes, and named them Ad-P450 cells. The activity levels of P450s in Ad-P450 cells and human hepatocytes were calculated via simultaneous liquid chromatography/tandem mass spectrometry analysis utilizing a P450 substrate cocktail. We established Ad-P450 cells mimicking the activity levels of CYP...Continue Reading

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Citations

Nov 9, 2017·International Journal of Molecular Sciences·Christopher Trent Brewer, Taosheng Chen
Sep 20, 2019·International Journal of Molecular Sciences·Patricia Rodríguez CastañoAmit V Pandey
Sep 24, 2019·Clinical Pharmacology and Therapeutics·Charity NofzigerAndrea Gaedigk
Jul 28, 2020·Biotechnology and Applied Biochemistry·Sheila J SadeghiGianfranco Gilardi
May 7, 2020·Archives of Toxicology·Si ChenLei Guo
Jul 24, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kenichiro TodorokiToshimasa Toyo'oka
Nov 5, 2020·Genes·Christopher TaylorRichard M Turner
Jan 23, 2021·Critical Reviews in Food Science and Nutrition·Nicholas John Sadgrove
Jul 14, 2020·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Amanda K Leonberg-YooJennifer Trofe-Clark
Apr 17, 2021·Nature Communications·Simon Bo JensenNikos S Hatzakis

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