PMID: 2506904Sep 1, 1989Paper

Effect of heat on extracted HIV viral infectivity and antibody activity using the filter paper technique of blood sampling

AIDS
B EvengårdE Linder

Abstract

The diagnostic potential of the standardized filter paper technique at an outpatient clinic was further evaluated for individuals infected with HIV; the inactivation of HIV in dried blood from filter paper was also investigated. Heat treatment at 56 degrees C or 70 degrees C for 1 h did not demonstrably affect antibody activity as measured by enzyme-linked immunosorbent assay and Western blot. Prolonged treatment up to 18 h reduced the activity to some extent. Drying at room temperature of filter papers which had been soaked with HIV was sufficient to inactivate low, but not high, amounts of infectious virus. After exposure of the filter paper discs to 56 degrees C or 70 degrees C for 1 h, HIV could not be isolated from the extracts. The obvious advantage of blood collection on filter paper for serological studies makes it an alternative to venipuncture, especially when the blood might be contagious for HIV.

Citations

Jan 9, 2004·Diagnostic Microbiology and Infectious Disease·A ChakravartiSangita Yadav
Sep 6, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Asa JanssonRajaa A Mirghani
Mar 8, 2005·AIDS·Jean-Christophe PlantierFrançois Simon
Feb 3, 2000·Journal of Clinical Pathology·S P Parker, W D Cubitt
Dec 25, 2013·The American Journal of Tropical Medicine and Hygiene·Pieter W SmitPaul N Newton
Oct 11, 2008·International Journal of Pharmaceutics·Hirotoshi UtsunomiyaTsutomu Arakawa
Jan 22, 2009·Biotechnology Journal·Tsutomu ArakawaA Hajime Koyama
Jan 1, 1989·Scandinavian Journal of Infectious Diseases. Supplementum·B Evengård
Apr 10, 2003·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Julian D DruceJoseph J Smolich
Apr 29, 2020·Diagnostics·Kristina MalsagovaAnna Kaysheva
Aug 14, 2019·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Barsa Baisalini PandaRupenangshu Kumar Hazra

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