Effect of Heat Processing on IgE Reactivity and Cross-Reactivity of Tropomyosin and Other Allergens of Asia-Pacific Mollusc Species: Identification of Novel Sydney Rock Oyster Tropomyosin Sac g 1

Molecular Nutrition & Food Research
J M RollandR E O'Hehir

Abstract

Shellfish allergy is an increasing global health priority, frequently affecting adults. Molluscs are an important shellfish group causing food allergy but knowledge of their allergens and cross-reactivity is limited. Optimal diagnosis of mollusc allergy enabling accurate advice on food avoidance is difficult. Allergens of four frequently ingested Asia-Pacific molluscs are characterized: Sydney rock oyster (Saccostrea glomerata), blue mussel (Mytilus edulis), saucer scallop (Amusium balloti), and southern calamari (Sepioteuthis australis), examining cross-reactivity between species and with blue swimmer crab tropomyosin, Por p 1. IgE ELISA showed that cooking increased IgE reactivity of mollusc extracts and basophil activation confirmed biologically relevant IgE reactivity. Immunoblotting demonstrated strong IgE reactivity of several proteins including one corresponding to heat-stable tropomyosin in all species (37-40 kDa). IgE-reactive Sydney rock oyster proteins were identified by mass spectrometry, and the novel major oyster tropomyosin allergen was cloned, sequenced, and designated Sac g 1 by the IUIS. Oyster extracts showed highest IgE cross-reactivity with other molluscs, while mussel cross-reactivity was weakest. Inhibiti...Continue Reading

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Citations

Nov 30, 2018·Expert Review of Clinical Immunology·Agnes Sze Yin LeungGary Wing Kin Wong
Jan 8, 2021·Clinical Reviews in Allergy & Immunology·Joana CostaThomas Holzhauser
Mar 21, 2019·Current Opinion in Allergy and Clinical Immunology·Lydia WongBee Wah Lee
Jan 13, 2021·The Journal of Allergy and Clinical Immunology. in Practice·Amanda L CoxScott H Sicherer
Jan 18, 2020·The Journal of Allergy and Clinical Immunology. in Practice·Carla M DavisAndreas L Lopata
Apr 17, 2020·Journal of Agricultural and Food Chemistry·Tian-Jiao HanGuang-Ming Liu
Aug 25, 2021·Molecular Biology Reports·Nur Farah Hani AzemiHaidr Msahir Ateshan

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Datasets Mentioned

BETA
AB444943.1
MF996471

Methods Mentioned

BETA
ELISA
flow cytometry
PCR

Software Mentioned

Mascot
GraphPad Prism

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