Effect of Heat Stress on Sperm DNA: Protamine Assessment in Ram Spermatozoa and Testicle

Oxidative Medicine and Cellular Longevity
Thais Rose dos Santos HamiltonMayra Elena Ortiz D Ávila Assumpção

Abstract

Sperm DNA fragmentation is considered one of the main causes of male infertility. The most accepted causes of sperm DNA damage are deleterious actions of reactive oxygen species (ROS), defects in protamination, and apoptosis. Ram sperm are highly prone to those damages due to the high susceptibility to ROS and to oxidative stress caused by heat stress. We aimed to evaluate the effects of heat stress on the chromatin of ejaculated and epididymal sperm and the activation of apoptotic pathways in different cell types in ram testis. We observed higher percentages of ejaculated sperm with increased chromatin fragmentation in the heat stress group; a fact that was unexpectedly not observed in epididymal sperm. Heat stress group presented a higher percentage of spermatozoa with DNA fragmentation and increased number of mRNA copies of transitional protein 1. Epididymal sperm presented greater gene expression of protamine 1 on the 30th day of the spermatic cycle; however, no differences in protamine protein levels were observed in ejaculated sperm and testis. Localization of proapoptotic protein BAX or BCL2 in testis was not different. In conclusion, testicular heat stress increases ram sperm DNA fragmentation without changes in protami...Continue Reading

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Citations

Mar 29, 2019·Andrology·T R S HamiltonM E O A Assumpção
Oct 12, 2019·Zygote : the Biology of Gametes and Early Embryos·Thais Rose Dos Santos Hamilton, Mayra Elena Ortiz D'Ávila Assumpção
May 10, 2020·Reproduction in Domestic Animals = Zuchthygiene·Marwa El-ZeftawyMervat Hassan
May 11, 2020·International Journal of Biometeorology·Laura Nataly Garcia-OliverosEneiva Carla Carvalho Celeghini
Aug 11, 2020·The World Journal of Men's Health·Ashok AgarwalRupin Shah
Aug 14, 2020·Reproductive Biology and Endocrinology : RB&E·Yan-Qing WuWei Xia
Aug 20, 2019·Zygote : the Biology of Gametes and Early Embryos·Thais Rose Dos Santos HamiltonMayra Elena Ortiz D'Ávila Assumpção
Jul 27, 2021·Andrologia·Zohreh Talebi-YazdabadiMohammad Hossein Nasr-Esfahani
Jul 8, 2021·Reproductive Sciences·Maryam PiraniZahra Shams Mofarahe

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Methods Mentioned

BETA
flow cytometry
Assay
PCR
electrophoresis
protein assay

Software Mentioned

Statistical Analysis System
SAS
BLAST
NIS Elements®
Primer
Image Lab

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