Effect of heat treatment on the antigen-binding activity of anti-peroxidase immunoglobulins in bovine colostrum

Journal of Dairy Science
E DomínguezM Calvo

Abstract

After intramammary immunization with horseradish peroxidase, bovine colostrum containing anti-peroxidase immunoglobulins (Ig) was obtained. Thermoresistance of the antigen-binding region of these Ig was studied using a direct competitive ELISA. This technique is based on the competition between the anti-peroxidase IgG coated to the plate and the anti-peroxidase IgG contained in the colostrum to bind peroxidase, the antigen, and the enzyme responsible for development of the color of the assay. Thus, the degree of denaturation of the antigen-binding region in the IgG molecule can be determined because this region is directly involved in the assay. The kinetic and thermodynamic parameters for heat induced denaturation of IgG in colostrum were determined over a temperature range of 69 to 81 degrees C. The denaturation of IgG was best described assuming an apparent reaction order of 1.5. D values, the time required to reduce the antigen-binding activity of IgG by 90%, were 8504, 1387, 285, and 152 s at 69, 72, 77, and 81 degrees C, respectively. Similarly, Z value, the degrees necessary to reduce the D value in one logarithmic cycle, was estimated to be 6.6 degrees C, and the activation energy value was 386.83 kJ/mol. These results ...Continue Reading

References

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Citations

Nov 11, 2008·Foodborne Pathogens and Disease·B A HouserB M Jayarao
Jan 19, 2012·Nutrients·Walter L Hurley, Peter K Theil
Jun 21, 2007·Analytical and Bioanalytical Chemistry·Leyton W GapperHarvey E Indyk
Mar 10, 2001·The British Journal of Nutrition·H KorhonenH S Gill
Jul 14, 2011·The Protein Journal·V Sathya DeviJeremy Truntzer
Oct 10, 2013·Journal of the Science of Food and Agriculture·Francisco RieraFernando Vicente
Feb 28, 2013·Journal of Animal Physiology and Animal Nutrition·P BalanP J Moughan
Jul 1, 2020·Journal of Clinical Laboratory Analysis·Xiumei HuLei Zheng
Oct 26, 2011·Analytica Chimica Acta·Thomas MoreauOdile Vandenabeele-Trambouze
Feb 3, 2005·Journal of Agricultural and Food Chemistry·Si-Quan LiQ Howard Zhang

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