Effect of Helicobacter pylori cdrA on interleukin-8 secretions and nuclear factor kappa B activation.

World Journal of Gastroenterology : WJG
Hiroaki TakeuchiTetsuro Sugiura

Abstract

To investigate genetic diversity of Helicobacter pylori (H. pylori) cell division-related gene A (cdrA) and its effect on the host response. Inactivation of H. pylori cdrA, which is involved in cell division and morphological elongation, has a role in chronic persistent infections. Genetic property of H. pylori cdrA was evaluated using polymerase chain reaction and sequencing in 128 (77 American and 51 Japanese) clinical isolates obtained from 48 and 51 patients, respectively. Enzyme-linked immunosorbent assay was performed to measure interleukin-8 (IL-8) secretion with gastric biopsy specimens obtained from American patients colonized with cdrA-positive or -negative strains and AGS cells co-cultured with wild-type HPK5 (cdrA-positive) or its derivative HPKT510 (cdrA-disruptant). Furthermore, the cytotoxin-associated gene A (cagA) status (translocation and phosphorylation) and kinetics of transcription factors [nuclear factor-kappa B (NF-κB) and inhibition kappa B] were investigated in AGS cells co-cultured with HPK5, HPKT510 and its derivative HPK5CA (cagA-disruptant) by western blotting analysis with immunoprecipitation. Genetic diversity of the H. pylori cdrA gene demonstrated that the cdrA status segregated into two categor...Continue Reading

References

Aug 1, 1992·Journal of Medical Microbiology·K A EatonS Krakowka
Jun 1, 1988·Journal of Medical Microbiology·R D LeunkD R Morgan
Oct 1, 1994·Journal of Clinical Pathology·J E CrabtreeD S Tompkins
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A CovacciN Figura
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·S SuerbaumM Achtman
Nov 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·D A IsraelR M Peek
Mar 21, 2002·Nature Reviews. Cancer·Richard M Peek, Martin J Blaser
May 2, 2002·Cell·Sankar Ghosh, Michael Karin
Jan 7, 2003·The Journal of Biological Chemistry·Naohiro InoharaGabriel Nuñez
Jul 27, 2004·Science·Marina Aspholm-HurtigThomas Borén
Oct 19, 2004·Nature Immunology·Jérôme VialaRichard L Ferrero
Jun 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jung D OhJeffrey I Gordon
Sep 15, 2006·The European Respiratory Journal·B SchmeckS Hippenstiel
Jun 15, 2007·Biochemical and Biophysical Research Communications·Jing MiBryan M Clary
Apr 26, 2008·Journal of Medical Microbiology·Yoshio Yamaoka
Aug 23, 2008·Gene·Joshua R Leeman, Thomas D Gilmore
Oct 28, 2008·Journal of Bacteriology·David A BaltrusKaren Guillemin
Nov 7, 2009·Journal of Agricultural and Food Chemistry·Vu Thu TrangHiroyuki Ukeda
Nov 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Do Jin KimSe Won Suh

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Citations

Feb 11, 2016·FEMS Microbiology Letters·Yoshie NishidaTetsuro Sugiura
Sep 14, 2012·Helicobacter·Alojz IhanEllen J Beswick
Jan 22, 2013·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Luca ValentiStefano Romeo
May 9, 2018·Microbiology·Hiroaki TakeuchiYoshihisa Matsumura

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