Effect of hemin and Protoporphyrin IX on the protein-synthesizing activity of human granulocytes, lymphocytes and platelets

Acta Haematologica
Z MalikM Djaldetti

Abstract

The hemin effect on protein synthesis of human granulocytes, lymphocytes and platelets was examined. Hemin added to culture media without serum caused a dose-dependent inhibition of protein synthesis in all three cell types. A cell-specific enhancement of protein-synthesizing capability was observed in 24-hour cultures in the presence of hemin and serum. A marked increase of protein synthesis was found in granulocytes, unchanged in lymphocytes and decreased in platelets. Lymphocytes from patients with chronic lymphatic leukemia (CLL) were moderately inhibited by hemin when incubated in media containing serum, the effect being more pronounced in the presence of freshly disolved doses of hemin. Addition of protoporphyrin IX to cells cultures resulted in a marked suppression of protein synthesis by the three cell types, in all experiments. These results confirm the importance of serum proteins in preventing the inhibitory effects of free hemin and protoporphyrin IX on blood cell protein synthesis. On the other hand, they show a cell-specific enhancement of the protein-synthesizing capacity mediated by hemin.

Citations

Dec 12, 1985·Biochimica Et Biophysica Acta·G AgamA Livne
Nov 6, 2008·Journal of Thrombosis and Haemostasis : JTH·A S WeyrichG A Zimmerman
Dec 1, 1988·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R Vassilopoulou-Sellin, C O Oyedeji
Jan 1, 2016·Zhurnal voprosy neĭrokhirurgii imeni N. N. Burdenko·V A ShurkhayA A Potapov
Oct 15, 1980·International Journal of Cancer. Journal International Du Cancer·Z Malik, M Djaldetti
Nov 1, 1995·Photochemistry and Photobiology·Q PengK E Giercksky

Related Concepts

Blood Platelets
Serum Proteins
Granulocyte
Heme
Panhematin
Lymphoid Cells
Porphyrins
Protoporphyrins
In Vitro [Publication Type]

Related Feeds

B-Cell Leukemia (Keystone)

B-cell leukemia includes various types of lymphoid leukemia that affect B cells. Here is the latest research on B-cell leukemia.