PMID: 9443579Jul 1, 1997Paper

Effect of hepatitis B virus on tumour necrosis factor (TNF alpha) gene expression in human THP-1 monocytic and Namalwa B-cell lines

Research in Immunology
J OquendoM A Petit

Abstract

In response to viruses, monocytes and B cells produce TNF alpha. Therefore, we investigated TNF alpha gene expression and protein secretion in a human monocytic cell line, THP-1, and a Burkitt's lymphoma B-cell line, Namalwa, in response to hepatitis B virus (HBV). Stimulation by phorbol myristate acetate (PMA) (100 ng/ml for 48 h) induced TNF alpha secretion in THP-1 and Namalwa cells (100 to 300 pg/ml). In THP cells, the optimum response (> 2000 pg/ml) was obtained in the presence of a second mitogenic signal such as lipopolysaccharide (LPS) (10 microg/ml for 24 h). In our activation conditions, Northern blot analysis revealed a marked accumulation of TNF alpha mRNA species at 1.7 kb in both cell lines. When PMA- or PMA+LPS-stimulated THP-1 cells were exposed to HBV, TNF alpha protein and mRNA significantly decreased (> 50%). In contrast, HBV exposure of PMA-activated Namalwa cells resulted in strongly increased TNF alpha protein secretion (1 ng/ml). In this case, HBV induced TNF alpha mRNA accumulation that consisted of two types: a regular 1.7 kb and two novel high molecular weight (HMW) species at 3.7 and 4.3 kb. Exposure of stimulated THP-1 and Namalwa cells to HBV resulted in HBs and pre-S1 antigen production in the supe...Continue Reading

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Citations

Oct 10, 2008·Journal of Virology·Mathis Heydtmann
Mar 10, 2001·Microbiology and Molecular Biology Reviews : MMBR·T H Mogensen, S R Paludan
Oct 2, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E Lara-PezziM López-Cabrera
Feb 22, 2002·Medicinal Research Reviews·Matthew D Vera, Madeleine M Joullié
Jan 16, 2021·Journal of Clinical and Translational Hepatology·Yujia LiLimin Chen

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