Effect of hepatocyte proliferation and cellular DNA synthesis on hepatitis B virus replication

Gastroenterology
A OzerB Yoffe

Abstract

Interrelationship between hepatitis B virus (HBV) replication and the stage of hepatocyte proliferation and differentiation may play an important role in the pathogenesis of HBV infection. The aim of this study was to assess the effect of hepatocyte proliferation and/or cell arrest on HBV replication. Hepatoblastoma cells transfected with HBV were subjected to serum deprivation or treatment with aphidicolin or camptothecin. Cell cycle analysis was performed using flow cytometry, and cellular DNA synthesis was analysed by assessing 5-bromo-2'-deoxyuridine incorporation. Distribution of episomal HBV DNA and proliferating cell nuclear antigen in liver specimens was assessed by simultaneous in situ hybridization and immunohistochemistry. Serum deprivation inhibited cellular DNA synthesis and increased levels of HBV messenger RNA (mRNA). Aphidicolin treatment resulted in cell arrest in C1, with concomitant increases in levels of HBV mRNA and viral DNA. Cell entry into S phase inhibited expression of HBV mRNA. Camptothecin induced G2 cell arrest and inhibited cellular DNA synthesis with increased amounts of viral replication and levels of HBV mRNA. In vivo studies showed an inverse correlation between expression of proliferating cell...Continue Reading

Citations

Feb 5, 1999·Journal of Hepato-biliary-pancreatic Surgery·M A Feitelson
Nov 16, 2004·Journal of Virology·Michael J Bouchard, Robert J Schneider
Jan 3, 2012·Journal of Biomedical Science·Chin-Liew ChongChungming Chang
Mar 31, 2011·World Journal of Gastroenterology : WJG·Ran ZhaoXiao-Ming Jin
Sep 30, 2009·Virus Research·Ruth ChinJoseph Torresi
May 27, 2006·Journal of Gastroenterology and Hepatology·Yusuf UzunOzden Uzunalimoglu
Dec 6, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Anja MeierStephan Urban
Jun 16, 2010·Journal of Viral Hepatitis·M Quasdorff, U Protzer
Apr 27, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Xiaoyong ZhangMengji Lu
Mar 8, 2014·Journal of Medical Virology·Sayeh EzzikouriKyoko Tsukiyama-Kohara
Aug 11, 2000·Seminars in Cancer Biology·C BréchotP Paterlini-Bréchot
May 5, 2016·Journal of Agricultural and Food Chemistry·Mengmeng ZhangTian Min
Jun 1, 2005·Journal of Hepatology·Beate FriedrichEberhard Hildt
Sep 25, 1999·Journal of Cellular Physiology·M A Feitelson
Jan 4, 1998·Journal of Gastroenterology and Hepatology·C M Chu, Y F Liaw
Oct 20, 2017·Viruses·Andoni Gómez-Moreno, Urtzi Garaigorta
Nov 5, 1999·In Vitro Cellular & Developmental Biology. Animal·V I KhaoustovB Yoffe
Jun 18, 2009·Journal of Mathematical Biology·Sarah HewsYang Kuang
Apr 7, 1999·Journal of Clinical Gastroenterology·J GalskýJ Kõnig
Jun 3, 2020·Nature Communications·Carla EllerThomas F Baumert

❮ Previous
Next ❯

Related Concepts

Related Feeds