Effect of herpesvirus infection on pancreatic duct cell secretion

World Journal of Gastroenterology : WJG
Péter HegyiZsolt Boldogköi


To examine the effect of acute infection caused by herpesvirus (pseudorabies virus, PRV) on pancreatic ductal secretion. The virulent Ba-DupGreen (BDG) and non-virulent Ka-RREp0lacgfp (KEG) genetically modified strains of PRV were used in this study and both of them contain the gene for green fluorescent protein (GFP). Small intra/interlobular ducts were infected with BDG virus (10(7) PFU/mL for 6 h) or with KEG virus (10(10) PFU/mL for 6 h), while non-infected ducts were incubated only with the culture media. The ducts were then cultured for a further 18 h. The rate of HCO(3)(-) secretion (base efflux -J(B-)) was determined from the buffering capacity of the cells and the initial rate of intracellular acidification (1) after sudden blockage of basolateral base loaders with dihydro-4,4-diisothiocyanatostilbene-2,2-disulfonic acid (500 micromol/L) and amiloride (200 micromol/L), and (2) after alkali loading the ducts by exposure to NH(4)Cl. All the experiments were performed in HCO(3)(-)-buffered Ringer solution at 37 degrees (n = 5 ducts for each experimental condition). Viral structural proteins were visualized by immunohistochemistry. Virally-encoded GFP and immunofluorescence signals were recorded by a confocal laser scannin...Continue Reading


Jul 25, 2015·Clinics and Research in Hepatology and Gastroenterology·Anita Balázs, Péter Hegyi
Sep 8, 2010·The American Journal of Gastroenterology·Péter Hegyi, Zoltán Rakonczay

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Carbonic Acid Ions
Cavia porcellus
Structure of Accessory Pancreatic Duct
Herpesvirus 1, Suid
Green Fluorescent Proteins
In Vitro [Publication Type]

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