Effect of herpesvirus infection on pancreatic duct cell secretion

World Journal of Gastroenterology : WJG
Péter HegyiZsolt Boldogköi

Abstract

To examine the effect of acute infection caused by herpesvirus (pseudorabies virus, PRV) on pancreatic ductal secretion. The virulent Ba-DupGreen (BDG) and non-virulent Ka-RREp0lacgfp (KEG) genetically modified strains of PRV were used in this study and both of them contain the gene for green fluorescent protein (GFP). Small intra/interlobular ducts were infected with BDG virus (10(7) PFU/mL for 6 h) or with KEG virus (10(10) PFU/mL for 6 h), while non-infected ducts were incubated only with the culture media. The ducts were then cultured for a further 18 h. The rate of HCO(3)(-) secretion (base efflux -J(B-)) was determined from the buffering capacity of the cells and the initial rate of intracellular acidification (1) after sudden blockage of basolateral base loaders with dihydro-4,4-diisothiocyanatostilbene-2,2-disulfonic acid (500 micromol/L) and amiloride (200 micromol/L), and (2) after alkali loading the ducts by exposure to NH(4)Cl. All the experiments were performed in HCO(3)(-)-buffered Ringer solution at 37 degrees (n = 5 ducts for each experimental condition). Viral structural proteins were visualized by immunohistochemistry. Virally-encoded GFP and immunofluorescence signals were recorded by a confocal laser scannin...Continue Reading

Citations

Jul 25, 2015·Clinics and Research in Hepatology and Gastroenterology·Anita Balázs, Péter Hegyi
Sep 8, 2010·The American Journal of Gastroenterology·Péter Hegyi, Zoltán Rakonczay

Related Concepts

Metazoa
Carbonic Acid Ions
Cavia porcellus
Structure of Accessory Pancreatic Duct
Pseudorabies
Herpesvirus 1, Suid
Green Fluorescent Proteins
In Vitro [Publication Type]

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