PMID: 8452523Mar 1, 1993Paper

Effect of heterologous expression of acyl-CoA-binding protein on acyl-CoA level and composition in yeast

The Biochemical Journal
S MandrupJ Knudsen

Abstract

We have expressed a bovine synthetic acyl-CoA-binding protein (ACBP) gene in yeast (Saccharomyces cerevisiae) under the control of the GAL1 promoter. The heterologously expressed bovine ACBP constituted up to 6.4% of total cellular protein and the processing was identical with that of native bovine ACBP, i.e. the initiating methionine was removed and the following serine residue was N-acetylated. The expression of this protein did not affect the growth rate of the cells. Determination of the yeast acyl-CoA pool size showed a close positive correlation between the ACBP content of the cells and the size of the acyl-CoA pool. Thus ACBP can act as an intracellular acyl-CoA pool former. Possible physiological functions of ACBP in cells are discussed.

Citations

Jun 9, 1993·Molecular and Cellular Biochemistry·J KnudsenK Kristiansen
Sep 1, 1996·Lipids·R E GossettF Schroeder
Aug 11, 2005·American Journal of Physiology. Endocrinology and Metabolism·Liping HuBarbara E Corkey
May 24, 2003·Applied Microbiology and Biotechnology·Y WachéJ-M Belin
May 16, 2008·Archives of Insect Biochemistry and Physiology·Jia-Lin WangXiao-Fan Zhao
Nov 26, 2010·The Journal of Biological Chemistry·Ditte NeessSusanne Mandrup
Dec 15, 2015·Lipids·Zi-Wei Ye, Mee-Len Chye
Jan 29, 2020·The Plant Journal : for Cell and Molecular Biology·Yang XuGuanqun Chen
Apr 25, 2000·The Journal of Biological Chemistry·K G Milne, M A Ferguson
Sep 3, 1998·The Journal of Biological Chemistry·S MandrupK Kristiansen
Mar 1, 1996·The Proceedings of the Nutrition Society·P BesnardH Carlier
Oct 3, 2006·Molecular and Cellular Biochemistry·Nils J FaergemanJens Knudsen
Mar 3, 2015·Molecular Endocrinology·Vimal SelvarajLan N Tu
Jul 17, 2016·The Journal of Endocrinology·Vimal Selvaraj, Lan N Tu
Jun 9, 2016·Applied Microbiology and Biotechnology·Matías CabrujaGabriela Gago
Jul 10, 2019·Pharmacology & Therapeutics·Marie-Christine TononJérôme Leprince
Mar 21, 1998·Archives of Biochemistry and Biophysics·R E GossettF Schroeder
Oct 26, 1999·Biochemical and Biophysical Research Communications·R FulceriA Benedetti
Jan 1, 1995·Progress in Lipid Research·J H Veerkamp, R G Maatman
Mar 27, 2001·Insect Biochemistry and Molecular Biology·S MatsumotoS Tatsuki
Jan 1, 1996·Progress in Lipid Research·R Lehner, A Kuksis
Mar 1, 1997·Progress in Lipid Research·P A Watkins
Dec 9, 1994·Biochimica Et Biophysica Acta·P Moreau, C Cassagne
Aug 12, 2006·The Journal of Investigative Dermatology·Lance LeeMark D Fleming

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