PMID: 20650072Apr 1, 1995Paper

Effect of high polyol concentrations on the neutral red absorption assay and tetrazolium-MTT test of rat hepatocytes in primary culture

Toxicology in Vitro : an International Journal Published in Association with BIBRA
P OlivierD Abbott

Abstract

The effects of three polyols, mannitol, sorbitol and xylitol, on primary cultures of rat hepatocytes were studied with the aid of two cytotoxicity markers, the MTT test and the neutral red assay. A dose-related increase in the uptake of neutral red (up to approximately 60%) was observed in comparison with untreated cells, whereas the MTT test was unaffected by concentrations of polyols up to 330 mM (620 mOsmol/litre). The increase in neutral red uptake does not indicate cellular toxicity as it was reversible and was shown to be due to an increase in the size of lysosomes. For compounds acting on cellular metabolism, such as phenol, mercuric chloride and pentobarbital, it was shown that the increase in neutral red uptake may minimize any cytotoxic effect of these compounds. The neutral red assay should therefore be used with caution when testing compounds that provoke lysosomal swelling. We recommend the use of multiple, mechanistically different, cytotoxicity markers for the evaluation of xenobiotic cytotoxicity.

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Citations

Apr 12, 2000·Toxicon : Official Journal of the International Society on Toxinology·N RakbaG Lescoat
Sep 21, 2000·Toxicology Letters·P da Silva MeloM Haun
Jun 1, 1998·Toxicology in Vitro : an International Journal Published in Association with BIBRA·K ChibaK Tohyama
Mar 25, 2016·European Journal of Dentistry·La-Ongthong Vajrabhaya, Suwanna Korsuwannawong
Apr 21, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Juan SunXiaohui Ouyang
Apr 28, 2004·European Journal of Medicinal Chemistry·Bok Young KimSeung Soo Yoon
Oct 13, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Nathalie DijouxPierre-Jacques Ferret
Oct 27, 2018·Journal of Veterinary Research·Sylwia TobólskaAndrzej Krzysztof Siwicki
Jul 12, 2019·Environmental and Molecular Mutagenesis·Daniel J SmartPatrick Vanscheeuwijck

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