Effect of high-pressure-induced ice I-to-ice III phase transitions on inactivation of Listeria innocua in frozen suspension

Applied and Environmental Microbiology
C LuscherD Knorr

Abstract

The inactivation of Listeria innocua BGA 3532 at subzero temperatures and pressures up to 400 MPa in buffer solution was studied to examine the impact of high-pressure treatments on bacteria in frozen matrices. The state of aggregation of water was taken into account. The inactivation was progressing rapidly during pressure holding under liquid conditions, whereas in the ice phases, extended pressure holding times had comparatively little effect. The transient phase change of ice I to other ice polymorphs (ice II or ice III) during pressure cycles above 200 MPa resulted in an inactivation of about 3 log cycles, probably due to the mechanical stress associated with the phase transition. This effect was independent of the applied pressure holding time. Flow cytometric analyses supported the assumption of different mechanisms of inactivation of L. innocua in the liquid phase and ice I (large fraction of sublethally damaged cells due to pressure inactivation) in contrast to cells subjected to ice I-to-ice III phase transitions (complete inactivation due to cell rupture). Possible applications of high-pressure-induced phase transitions include cell disintegration for the recovery of intracellular components and inactivation of micro...Continue Reading

References

Apr 1, 1976·Biotechnology and Bioengineering·K E Magnusson, L Edebo
Aug 1, 1995·Bioscience, Biotechnology, and Biochemistry·C HashizumeR Hayashi
Nov 27, 1998·Applied Microbiology and Biotechnology·K HayakawaR Hayashi
May 2, 2002·Biochimica Et Biophysica Acta·Shigeru Kunugi, Naoki Tanaka

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Citations

Sep 4, 2007·Applied and Environmental Microbiology·Marwen MoussaPatrick Gervais
Jul 3, 2013·International Journal of Biomaterials·S EichhornM Krane
Oct 7, 2015·Frontiers in Microbiology·Antje Fröhling, Oliver Schlüter
Apr 15, 2008·Critical Reviews in Food Science and Nutrition·B GuignonP D Sanz

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