Effect of holding time, temperature and different parenteral solutions on viability and functionality of adult bone marrow-derived mesenchymal stem cells before transplantation

Journal of Tissue Engineering and Regenerative Medicine
Rakhi PalSatish M Totey

Abstract

Mesenchymal stem cells (MSCs) have the ability to proliferate and differentiate into various lineages, given the appropriate microenvironment, thus making MSCs promising candidates for cell transplantation. For clinical applications, MSCs need to be stored in optimal conditions so that they may be transported and made available as an off-the-shelf product for companies to market. Freshly harvested and cultured or frozen-thawed bone marrow-derived MSCs were prepared for cell transplantation. Both freshly cultured or frozen-thawed MSCs were washed and resuspended in parenteral solutions, either 0.9% saline, Dulbecco's phosphate-buffered saline (DPBS), plasmalyte A or 5%dextrose and held for 2, 4, 6 and 8 h at 4 degrees C, 37 degrees C and RT (22 degrees C). The viability of the cells, differentiation capability and expression of cell surface markers were analysed. MSCs harvested from fresh cultures, resuspended in the parenteral solutions and maintained at 4 degrees C for 6 h showed more than 90% viability, and the viability was appreciably better when suspended in 5% dextrose at 4 degrees C for 8 h. In contrast, frozen-thawed cells can be held for a maximum of 2 h after thawing before losing their viability significantly below p...Continue Reading

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Feb 11, 2014·BioMed Research International·Chiho Ikebe, Ken Suzuki
Feb 15, 2011·Stem Cells International·Karen BiebackMarianna Karagianni
Mar 26, 2013·Archives of Medical Research·Ali Asghar KianiMehryar Habibi Roudkenar
Dec 16, 2011·Journal of Cellular Physiology·Guohu DiHaifeng Duan
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Aug 12, 2014·Stem Cell Research & Therapy·Elaine R GarvicanJayesh Dudhia
Jun 25, 2021·Stem Cell Research & Therapy·Iman Razeghian-JahromiJerome Roncalli

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