PMID: 4897124Jul 1, 1969Paper

Effect of host cell wall material on the adsorbability of cofactor-requiring T4

Journal of Virology
D T Brown, T F Anderson

Abstract

The adsorbability of T4 on host cells was determined as a function of time after their liberation from infected cells. Freshly liberated (nascent) particles are readily adsorbed but lose their adsorbability with a half-time of about 2 days at 5 C, but only about 20 min at 37 C. They can be made adsorbable again with an alpha-amino acid cofactor like l-tryptophan, and this state of adsorbability can be stabilized by cell wall material from Escherichia coli. Such stabilized particles lose their adsorbability at a rate similar to that at which nascent particles lose theirs. Most freshly liberated particles are observed by means of electron microscopy to have "debris" attached to their baseplates and to have most of their six, long tail fibers free, whereas "old" particles that have lost their adsorbability appear relatively "clean" with most of their tail fibers wrapped around their sheaths. Nascent particles have densities that are lower than those of old particles. The material responsible for nascent adsorbability seems to be a fragment of the host's cell wall, for nascent adsorbability is destroyed by lysozyme. Furthermore, nascent T4 particles liberated from host cells with radioactively labeled walls carry the label in densi...Continue Reading

References

Jan 1, 1968·Journal of Virology·R W Darlington, L H Moss
Jan 1, 1966·Annual Review of Biochemistry·H H Martin
May 1, 1968·Journal of Virology·R I Gamow, L M Kozloff
Nov 1, 1952·Biochimica Et Biophysica Acta·E L WOLLMAN, G S STENT
Feb 1, 1964·Biochemistry·L C KANNER, L M KOZLOFF
Nov 1, 1950·Biochimica Et Biophysica Acta·E L WOLLMAN, G S STENT
May 1, 1948·Journal of Bacteriology·T F Anderson
Nov 15, 1957·Proceedings of the National Academy of Sciences of the United States of America·D FraserC A Thomas

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