PMID: 11930096Apr 4, 2002Paper

Effect of human milk and epidermal growth factor on growth of human intestinal Caco-2 cells

Journal of Pediatric Gastroenterology and Nutrition
Chun-Ju Chang, Jane C-J Chao

Abstract

Epidermal growth factor (EGF) in human milk has been thought to be mitogenic for cell growth. This study investigated the effects of human milk and EGF on the growth of human intestinal Caco-2 cells to determine whether the action occurred through regulation of the cell cycle or through c-jun expression. Cells were incubated with 5% human milk, 0.375 nmol/L EGF (relevant to EGF concentration in 5% human milk, 0.05 x EGF), 7.5 nmol/L EGF (1 x EGF), or 75 nmol/L EGF (10 x EGF). Cell numbers; cellular RNA, DNA, and protein concentrations; DNA content in the cell cycle, and expressions of c-Jun protein and mRNA were analyzed. Cell numbers increased in the 1 x and 10 x EGF groups at 48 hours. Cellular RNA increased in the 5% human milk and 10 x EGF groups. DNA and protein contents increased in the 1 x and 10 x EGF groups. The 1 x and 10 x EGF groups increased DNA content in the G1 phase compared with the 5% human milk group at 24 hours. The greatest c-jun protein expression was 2.6, 1.4, 1.8, and 1.9 times the control, and the c-jun mRNA increased by 202%, 14%, 150%, and 181%, respectively, in the 5% human milk, 0.05 x, 1 x, and 10 x EGF groups. In a dose-dependent manner, EGF stimulated intestinal growth in vitro, by increasing DNA...Continue Reading

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Citations

Apr 16, 2009·Breastfeeding Medicine : the Official Journal of the Academy of Breastfeeding Medicine·Peter B UntalanArmond S Goldman
Jul 21, 2009·Pediatric Research·Abdullah KumralYilmaz Ozalp
Nov 28, 2012·Pediatric Clinics of North America·Olivia Ballard, Ardythe L Morrow
Jun 22, 2005·World Journal of Gastroenterology : WJG·Hui LuXin-Dong Xue
Sep 8, 2018·The Cochrane Database of Systematic Reviews·Amna Widad A NasufJon Dorling
Apr 15, 2020·Nutrients·Katríona E LyonsCatherine Stanton

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