Effect of Human Platelet Lysate in Differentiation of Wharton's Jelly Derived Mesenchymal Stem Cells

Endocrine, Metabolic & Immune Disorders Drug Targets
Rosy VennilaSurendran Rajagopal

Abstract

Mesenchymal stem cells (MSCs) are highly preferred in clinical therapy for repair and regeneration of diseased tissues for their multipotent properties. Conventionally, MSCs have been cultured in media supplemented with animal derived serum, however, it is ideal to expand MSCs in media containing supplements of human origin for clinical therapy. Currently, a number of human derived products are being studied as an alternative to animal sources. Amongst these, platelet lysate (PL) has gained interest in the culture of MSCs without affecting their phenotypic property. In this study, we used various concentration of PL (2.5, 5, 7.5 & 10%) in the growth medium of MSCs to identify the least concentration of PL that could be an effective alternative to animal products. MSCs were isolated from Wharton's Jelly by using explant method and expanded in various concentration of PL supplemented medium against the standard FBS containing medium. WJ-MSCs were characterised as per the minimal criteria proposed by International Society for Cell therapy (ISCT), Proliferation study by BrdU assay, gene expression study by qRT-PCR, sterility test for bacteria, Mycoplasma by PCR and endotoxin detection by LAL assay. Whartons jelly derived MSCs (WJ-M...Continue Reading

References

May 12, 2005·Journal of Cellular Physiology·Christelle DoucetJean-Jacques Lataillade
Dec 25, 2009·Blood·Karin TarteUNKNOWN Société Française de Greffe de Moelle et Thérapie Cellulaire
Mar 2, 2010·The Journal of Surgical Research·Naoki MorimotoShigehiko Suzuki
Sep 15, 2010·Best Practice & Research. Clinical Haematology·Hadar Arien-ZakayArnon Nagler
Sep 21, 2012·World Journal of Stem Cells·Shalini VellasamyRajesh Ramasamy

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Citations

Dec 1, 2020·Platelets·Lassina BarroThierry Burnouf

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