Effect of human recombinant interleukin-6 and interleukin-8 on monocyte procoagulant activity

Arteriosclerosis, Thrombosis, and Vascular Biology
F J NeumannA Schömig

Abstract

Interleukin (IL)-6 and IL-8 are important regulators of inflammatory responses in myocardial infarction. Induction of monocyte procoagulant activity (PCA) by these cytokines could present a mechanism that links inflammatory responses to thrombotic events. We therefore investigated the effect of IL-6 and IL-8 on monocyte tissue factor (TF) expression. Recombinant human IL-6 and IL-8 caused a time- and dose-dependent increase in PCA (recalcification time) of monocytic U937 cells and of mononuclear leukocytes. Using blocking anti-TF monoclonal antibodies and factor VII-deficient control plasma, this PCA was shown to be TF dependent. Compared with unstimulated cells, mononuclear cell PCA increased by 4.5-fold to 17 +/- 2 mU/5x10(5) cells after exposure to 100 ng/L IL-6 for 4 hours and by 6.6-fold to 27 +/- 4 mU/5x10(5) cells after exposure to IL-8 under the same conditions. Northern blot analysis showed an increase in TF mRNA after stimulation with IL-6 or IL-8 for 2 hours, and after 4 hours an increase in cellular TF protein content was found by immunoassay. Flow cytometry demonstrated that IL-6 and IL-8 induced an increase in TF surface expression on monocytes. Thus, IL-6 and IL-8 induce monocyte PCA by increasing mRNA, protein c...Continue Reading

References

Mar 1, 1977·The Journal of Clinical Investigation·H RothbergerJ H Vaughan
Jul 1, 1975·British Journal of Haematology·R P RiversW L Weston
Dec 23, 1992·JAMA : the Journal of the American Medical Association·R C Bone
May 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A MantovaniE Dejana
Jun 1, 1992·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·S AlbrechtM Müller
Jan 1, 1991·Annual Review of Immunology·J J OppenheimK Matsushima
Mar 1, 1990·Immunology Today·E J Leonard, T Yoshimura
Jun 1, 1989·Molecular and Cellular Biology·S A GregoryT S Edgington
Aug 25, 1987·Biochemistry·E M ScarpatiJ E Sadler
Sep 1, 1985·The Journal of Clinical Investigation·G M VercellottiH S Jacob
Apr 1, 1988·Scandinavian Journal of Immunology·E Carlsen, H Prydz
Aug 18, 1994·The New England Journal of Medicine·G LiuzzoA Maseri
Aug 18, 1994·The New England Journal of Medicine·R W Alexander
Jan 1, 1996·The Journal of Surgical Research·T F RehringA H Harken

❮ Previous
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Citations

Nov 21, 2001·Clinical Cardiology·U IkedaK Shimada
Jan 8, 2004·Atherosclerosis·Michael HoffmanMoshe Benjamin
Mar 25, 2000·Neurochemistry International·B D KleinK S Callahan
Nov 28, 2001·British Journal of Haematology·R KerrC A Ludlam
Feb 14, 2002·British Journal of Haematology·Benien E van AkenFrits R Rosendaal
Dec 12, 2001·European Journal of Clinical Investigation·A J GrauC Grond-Ginsbach
Sep 26, 2003·The Proceedings of the Nutrition Society·Valerie A Holmes
Oct 3, 2012·Metabolic Syndrome and Related Disorders·Marinez Farana MatosVânia Maris Morelli
Sep 28, 2000·Critical Care Medicine·H ten Cate
Jun 12, 2004·Critical Care Medicine·Ulla DerhaschnigBernd Jilma
May 3, 2006·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Ken-ichiro InoueToshikazu Yoshikawa
Jul 27, 2004·Annals of Surgery·Fernando A Rivera-ChavezGrant E O'Keefe
Mar 15, 2006·European Journal of Haematology·Robert Bucki, Jennifer J Pastore
Feb 23, 2005·Occupational and Environmental Medicine·P S GilmourW MacNee
Apr 21, 2012·International Journal of Inflammation·Gabriele Demetz, Ilka Ott
May 9, 2013·International Journal of Inflammation·Avnish Deobhakta, Louis K Chang
Dec 6, 2001·American Journal of Respiratory and Critical Care Medicine·K E Welty-WolfC A Piantadosi
Sep 22, 2007·The Journal of Clinical Investigation·Gökhan M MutluG R Scott Budinger
Mar 1, 2009·Expert Opinion on Drug Discovery·Alexander D CornetAb Johan Groeneveld
Oct 7, 2015·Nature Reviews. Microbiology·Ilhem MessaoudiChristopher F Basler
Jul 7, 2016·Immunotherapy·Toshio TanakaTadamitsu Kishimoto
May 12, 2017·Expert Review of Clinical Immunology·Masashi NarazakiTadamitsu Kishimoto
Dec 6, 2006·Translational Research : the Journal of Laboratory and Clinical Medicine·Florian B Mayr, Bernd Jilma
Feb 13, 2001·The Journal of Pathology·B A LwaleedA J Cooper
Jan 9, 2010·Journal of Cardiothoracic and Vascular Anesthesia·Ian J WelsbyUNKNOWN Perioperative Genetics and Safety Outcomes Study Investigative Team
Mar 30, 2006·Atherosclerosis·Birgit A SteppichIlka Ott

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