PMID: 7012014Feb 1, 1981

Effect of human saliva on glucose uptake by Streptococcus mutans and other oral microorganisms

Infection and Immunity
G R Germaine, L M Tellefson

Abstract

We examined the effects of human whole salivary supernatant and parotid fluid on glucose uptake by Streptococcus mutans, Streptococcus sanguis, Streptococcus mitis, Actinomyces viscosus, Staphylococcus aureus, and Escherichia coli. The following three effects of saliva were observed: (i) inhibition of glucose uptake (S. mutans, S. sanguis), (ii) promotion of a transient, rapid (0 to 30 s) burst of glucose uptake (S. mutans, S. sanguis), and (iii) enhancement of glucose uptake (S. mitis, A. viscosus, S. aureus, E. coli). We observed no differences between the effects of whole salivary supernatant and the effects of parotid fluid. Heat treatment (80 degrees C, 10 min) of saliva or the addition of dithiothreitol abolished inhibition of glucose uptake. Supplementation of saliva with H(2)O(2) potentiated inhibition of glucose uptake. S. mitis and A. viscosus, which were stimulated by saliva alone, were inhibited by H(2)O(2)-supplemented saliva; 50% inhibition of glucose uptake by S. mutans and S. mitis required ca. 10 muM H(2)O(2) in 50% (vol/vol) saliva. Loss of the inhibitory action of saliva occurred at about 5% (vol/vol) saliva. Supplementation of saliva dilutions with SCN(-) and H(2)O(2) extended the inhibitory activity to solu...Continue Reading

Citations

Jun 1, 1987·Scandinavian Journal of Dental Research·N E FoudaM L Svensson
Dec 1, 1984·Journal of Oral Pathology·J Tenovuo, K M Pruitt
Jun 1, 1988·Oral Microbiology and Immunology·J TenovuoG Sievers
Oct 1, 1983·Journal of Dental Research·B Mansson-RahemtullaT M Le
Feb 1, 1985·Scandinavian Journal of Dental Research·G RöllaJ E Ciardi
Dec 15, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Tetsuya TanakaKei-Ichi Shimazaki

Related Concepts

Actinomyces
Sputolysin
Alkalescens-Dispar Group
Glucose, (beta-D)-Isomer
Oxydol
Vestibule of Mouth
Parotid Gland
Saliva
Staphylococcus aureus
Streptococcus

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