Effect of human sperm exposure to progesterone on sperm-oocyte fusion and sperm-zona pellucida binding under various experimental conditions

International Journal of Andrology
F FrancavillaS Francavilla

Abstract

In this study, the effect of human sperm exposure to progesterone on sperm/oocyte fusion, using the hamster egg penetration test, and on sperm/zona pellucida (ZP) binding, using the hemizona assay, was investigated under various experimental conditions. A brief exposure of human spermatozoa to progesterone exerted a stimulatory effect on sperm/oocyte fusion which was dose-dependent, capacitation-dependent, influenced by the source of serum albumin in capacitating medium, and was higher than that produced by the exposure to progesterone from the onset of capacitation. The exposure of capacitated spermatozoa to progesterone during 20 min-spermatozoa/ZP-coincubation produced an enhancement of ZP-binding, which was not significantly influenced by the source of serum albumin in capacitating medium. A significantly lower ZP-binding was exhibited by spermatozoa exposed to progesterone from the beginning of capacitation. These results indicate that progesterone exerts a stimulatory effect on human sperm's fertilizing ability, which occurs mainly in post-capacitation events directly involved in sperm/oocyte fusion and in ZP-binding. Conditions optimizing these effects are provided. They should be taken into account in the standardizatio...Continue Reading

References

Apr 28, 1989·Biochemical and Biophysical Research Communications·R A OsmanS Meizel
Feb 1, 1974·Journal of Reproduction and Fertility·D G Whittingham, W K Whitten
Mar 1, 1993·Fertility and Sterility·S E RavnikC H Muller
Feb 1, 1996·International Journal of Andrology·C EmiliozziP Fénichel
Feb 1, 1996·International Journal of Andrology·J YangP Jouannet
May 1, 1996·Biology of Reproduction·K SabeurS Meizel
Jul 23, 1998·Biology of Reproduction·N L Cross
Apr 15, 2011·Biology of Reproduction·Ryuzo Yanagimachi

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Citations

Mar 13, 2014·PloS One·Diego Rafael UñatesLaura Cecilia Giojalas
Feb 1, 2005·International Journal of Andrology·C PandolfiF Francavilla
May 18, 2007·Reproduction in Domestic Animals = Zuchthygiene·K LukoseviciuteA Januskauskas
Apr 14, 2016·Reproductive Sciences·Aideé Saray López-Torres, Mayel Chirinos
Oct 1, 2005·Alternatives to Laboratory Animals : ATLA·Susanne BremerHorst Spielmann
Feb 1, 2017·Reproductive Sciences·Aideé Saray López-Torres, Mayel Chirinos

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