Effect of Human Umbilical Cord Mesenchymal Stem Cell Transplantation in a Rat Model of Preeclampsia

Reproductive Sciences
Lei-Lei WangChong Qiao

Abstract

To test the effects of human umbilical cord mesenchymal stem cell (HU-MSC) transplantation on reversing preeclampsia (PE) symptoms in a lipopolysaccharide (LPS)-induced rat PE model. Human umbilical cord MSCs were detected, isolated, and cultured. Human umbilical cord MSC transplantation was conducted. Expressions of inflammatory cytokines in serum and placental tissue were measured by enzyme-linked immunosorbent assay. Changes in inflammatory cytokines, peroxisome proliferator-activated receptor γ (PPARγ), laminin receptor 1 (LR1), matrix metalloproteinase (MMP) 2, and MMP-9 messenger RNA (mRNA) levels in placental tissue were recorded by quantitative real-time polymerase chain reaction. Immunohistochemistry and Western blotting were performed for PPARγ detection. The LPS group exhibited increased blood pressure and proteinuria and decreased fetal weight compared to the normal pregnancy (NP) group (all P < .05). The LPS + MSC group presented lowered blood pressure and higher fetal weight than the LPS group (P < .05). The levels of interferon γ, tumor necrosis factor α (TNF-α), interleukin (IL) 1β, IL-6, IL-8, IL-12, and intercellular adhesion molecule 1 (ICAM-1) increased and the levels of IL-4 and IL-10 levels decreased in th...Continue Reading

References

Oct 21, 2004·Blood·Sudeepta Aggarwal, Mark F Pittenger
Mar 22, 2005·Experimental Cell Research·Ida RasmussonKatarina Le Blanc
Jun 20, 2007·Maternal and Child Health Journal·Luis O RustveldRavi Sharma
Oct 2, 2007·Cell Biology International·Chun QiaoYongchang Chen
Oct 24, 2007·Journal of Internal Medicine·K Le Blanc, O Ringdén
Feb 23, 2008·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·J A BrewsterJ J Walker
Mar 22, 2008·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Jennifer A BrewsterJames J Walker
Nov 4, 2008·Journal of Molecular Biology·Tsuyoshi WakuKosuke Morikawa
Dec 5, 2008·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sangmin KimJeong Eon Lee
Jan 15, 2009·Liver International : Official Journal of the International Association for the Study of the Liver·Yongmin YanFei Mao
Apr 29, 2009·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Pei-Chun TsaiYu-Show Fu
Jul 2, 2009·Cytotherapy·Jyoti A KodeAnandwardhan A Hardikar
Nov 24, 2011·Endocrinology and Metabolism Clinics of North America·Caren G Solomon, Ellen W Seely
Mar 1, 2012·The Biochemical Journal·Ashlee HigdonVictor M Darley-Usmar
Mar 22, 2012·Fetal and Pediatric Pathology·Katherine F MaloneyRebecca N Baergen
May 2, 2013·Journal of Leukocyte Biology·Estibalitz Laresgoiti-Servitje
Jul 3, 2013·The American Journal of Pathology·Drucilla J Roberts
Aug 7, 2013·Seminars in Perinatology·Gloria T Too, James B Hill
Aug 22, 2013·Best Practice & Research. Clinical Obstetrics & Gynaecology·N Arulkumaran, L Lightstone
Sep 21, 2013·Stem Cell Reports·William J AdamsGuillermo García-Cardeña
Oct 10, 2013·Advances in Experimental Medicine and Biology·Ossie Geifman-HoltzmanEliezer J Holtzman
Jan 15, 2014·Stem Cell Research & Therapy·Catharina De SchauwerGerlinde R Van de Walle
Feb 21, 2014·Tissue Engineering. Part B, Reviews·Reine El OmarVeronique Decot
May 8, 2014·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Marianne Joerger-MesserliAndreina Schoeberlein
May 9, 2014·American Journal of Reproductive Immunology : AJRI·Kelly K FergusonJohn D Meeker
Jun 10, 2014·Bulletin of Experimental Biology and Medicine·V N VeryasovG T Sukhikh
Jun 17, 2014·Laboratory Investigation; a Journal of Technical Methods and Pathology·Arjun JainChristiane Albrecht
Jul 17, 2014·Cellular & Molecular Immunology·Binqing FuHaiming Wei

❮ Previous
Next ❯

Citations

Dec 16, 2016·Clinical Obstetrics and Gynecology·Sara Ornaghi, Michael J Paidas
Feb 23, 2018·Journal of Molecular Endocrinology·Jonathan PhamMana M Parast
Dec 8, 2017·Reproductive Sciences·Maria Rosa Maduro
Aug 10, 2019·American Journal of Reproductive Immunology : AJRI·Guo-Qin LeiLi-Li Yu
May 11, 2019·BMC Pregnancy and Childbirth·Sophia GrimesDaniel J Corsi
Apr 16, 2020·Current Hypertension Reports·S SuvakovL McClements
May 2, 2018·Current Hypertension Reports·Hajrunisa CubroVesna D Garovic
Jul 4, 2020·The Journal of Clinical Endocrinology and Metabolism·Naomi ToddLana McClements
Oct 15, 2020·Cells·Sahar EsfandyariAyman Al-Hendy
Mar 10, 2021·Journal of Periodontal Research·Karina MataSergio Luiz Salvador
Jul 10, 2021·Mediators of Inflammation·Zengfang WangXue Liu
Aug 30, 2021·Advanced Drug Delivery Reviews·Anjali SharmaRangaramanujam M Kannan

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
Fluorescence
transfection
enzyme-linked immunosorbent assay
electrophoresis
X-ray
ELISA
PCR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.