Effect of hydroxylamine on photon-like events during dark adaptation in toad rod photoreceptors

The Journal of Physiology
C S Leibrock, T D Lamb

Abstract

1. The suction pipette technique was used to investigate the recovery of toad rod photoreceptors following small bleaches of 0.2-3% of the rhodopsin. 2. The reduction in sensitivity and the increase in noise elicited by bleaches were measured, and from these measurements the underlying rate of occurrence of photon-like events was calculated as a function of time after the bleach. 3. Exposure to hydroxylamine solution was used to hasten the decomposition of the metarhodopsin photoproducts. The outer segment was exposed to 110 mM hydroxylamine in a low-Ca2+ Ringer solution for a period of 10-50 s beginning 10-17 min after the bleaching exposure. 4. By the time of the hydroxylamine exposure, the flash sensitivity and response kinetics had returned almost to normal, and were not significantly altered by the exposure. 5. Following hydroxylamine exposure, the rate of spontaneous photon-like events in the rods declined rapidly to near pre-bleach levels. 6. We conclude that hydroxylamine reduces the rate of occurrence of photon-like events induced by a bleach, and we postulate that this reduction results from the removal of metarhodopsin (most likely metarhodopsin II) from the outer segment. 7. Our results are consistent with a model i...Continue Reading

References

Oct 1, 1977·The Journal of Physiology·K AzumaW Sickel
Apr 1, 1978·The Journal of General Physiology·D R PepperbergJ E Dowling
Jan 1, 1977·The Journal of General Physiology·K P Brin, H Ripps
Mar 1, 1992·Experimental Eye Research·D R Pepperberg, T I Okajima
Feb 1, 1989·Photochemistry and Photobiology·D OkadaA Ikai
Jan 1, 1985·The Journal of Physiology·A L HodgkinB J Nunn
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·U WildenH Kühn
Jan 1, 1967·Vision Research·K O Donner, T Reuter
Dec 1, 1984·The Journal of Physiology·D A BaylorJ L Schnapf
Dec 1, 1980·The Journal of Physiology·D A BaylorK W Yau
Jan 1, 1981·Vision Research·T D Lamb
Oct 15, 1994·The Journal of Physiology·M C Cornwall, G L Fain
Jan 1, 1995·Methods in Enzymology·D R PepperbergP J O'Brien
Nov 1, 1994·Vision Research·C S LeibrockT D Lamb
Jan 15, 1972·FEBS Letters·H Kühn, W J. Dreyer
Jul 1, 1963·The Journal of General Physiology·J E DOWLING
Nov 1, 1963·The Journal of General Physiology·R G MATTHEWSG WALD
Jul 20, 1937·The Journal of General Physiology·S HechtA M Chase

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Citations

Nov 9, 2000·Progress in Retinal and Eye Research·T Ebrey, Y Koutalos
Jun 8, 2001·Progress in Retinal and Eye Research·J K McBeeD R Pepperberg
May 2, 2012·The Journal of Biological Chemistry·Timothy J SextonRussell N Van Gelder
Sep 29, 2004·The Journal of General Physiology·Efthymia TsinaM Carter Cornwall
Jun 2, 2005·The Journal of General Physiology·David Holcman, Juan I Korenbrot
Nov 15, 2006·The Journal of General Physiology·Maureen E EstevezM Carter Cornwall
Jul 29, 2009·The Journal of General Physiology·Maureen E EstevezM Carter Cornwall
Jun 5, 2004·Progress in Retinal and Eye Research·T D Lamb, E N Pugh
Dec 8, 2004·Vision Research·M L FirsovV I Govardovskii
Oct 20, 2006·Vision Research·A V KolesnikovM C Cornwall
Sep 20, 2011·Vision Research·Tom Reuter
Jun 30, 2016·The Journal of General Physiology·Rikard FrederiksenM Carter Cornwall
Oct 17, 1998·Eye·C S LeibrockT D Lamb
Feb 24, 2019·Proceedings of the National Academy of Sciences of the United States of America·Wendy W S YueKing-Wai Yau
Jan 12, 2001·Physiological Reviews·G L FainY Koutalos

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