PMID: 7214029Mar 1, 1981

Effect of hydroxyurea on two different types of hematopoietic stem cells (CFU-S and DCPC) of newborn mice

Blut
Z Ben-IshayD Ben-Israel

Abstract

Pluripotent hematopoietic stem cells from the bone marrow of newborn mice (9--13 days of age) have been studied following the administration of hydroxyurea (HU, 1,000 mg/kg). Following a single injection as well as a regimen of eight injections complete bone marrow reconstitution was achieved after 7 days. During the recovery phase diffusion chamber progenitor cells (DCPC) from pretreated newborn mouse bone marrow proliferated strongly to give significantly higher cell yields than DCPC from control animals. By contrast (colony-forming units-spleen [CFU-S]) levels were reduced at the same time to between 15 and 40% of controls suggesting a small-sized reserve pool of resting CFU-S in newborn mice. Assuming that DCPC are less differentiated than CFU-S and are non-cycling stem cells they may possibly pass through the CFU-S stage rapidly in newborn mice under the stress of hematopoietic reconstitution and proceed directly to myelopoiesis.

Citations

Mar 1, 1976·Scandinavian Journal of Haematology·N J RencriccaF Stohlman
Jan 1, 1979·Cell and Tissue Kinetics·M RosendaalT R Bradley
Jan 1, 1979·Differentiation; Research in Biological Diversity·L G Lajtha
Jan 1, 1979·Nouvelle Revue Française D'hématologie·L G Lajtha
Oct 1, 1976·Proceedings of the Society for Experimental Biology and Medicine·A PorcelliniG Lucarelli
Mar 29, 1968·Annals of the New York Academy of Sciences·G LucarelliF Stohlman

Related Concepts

Protein S Deficiency
Colony-Forming Units, Hematopoietic
Spleen Colony-forming Unit
Myelopoiesis
Spleen
Bone Marrow
Cell Differentiation Process
Oncocarbide
Hydroxyurea
Spleen Tissue

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