PMID: 2493745Mar 1, 1989Paper

Effect of hypertonic stress on liver cell volume, bile flow, and volume-regulatory K+ fluxes

The American Journal of Physiology
P HaddadJ Graf

Abstract

Net hepatic uptake and release of K+ were studied in the isolated-perfused rat liver subjected to a 10-min period of hyperosmotic stress by addition of 80 mM mannitol or sucrose to the perfusing solution. Bile flow and effluent Na+, K+, and Ca2+ activities were monitored throughout. Upon initiation of hypertonic stress, a sharp transient dilution of effluent ion activities indicated hepatic water losses that were larger and occurred more rapidly with sucrose than with mannitol. During continuous hyperosmotic perfusion, portocaval differences in K+ uncovered a steady net influx of the ion into the liver that reached a higher maximum and led to a greater accumulation in experiments with sucrose compared with mannitol. This hepatic K+ uptake was completely blocked by 1 mM ouabain. Upon return to isotonic conditions, a sharp transient concentration of effluent ion activities suggested hepatic water uptake that was again more rapid and pronounced in sucrose- than mannitol-treated livers. This was followed by a transient phase of net hepatic K+ release whose magnitude and duration were proportional to the water movements induced by the removal of each carbohydrate. Administration of 2 mM Ba2+ abolished this K+ efflux. These results i...Continue Reading

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