Effect of hypoxia and TP53 mutation status and cytogenetics of normal and malignant mammary epithelium

Cancer Genetics and Cytogenetics
Hilmar VidarssonH M Ogmundsdóttir

Abstract

It has been proposed that hypoxia favors the growth of tumor cells over normal cells, particularly tumor cells carrying TP53 mutations. Cytogenetic studies of breast cancer have shown that highly complex karyotypes seen in direct harvest preparations are rarely detected after short-term culture. In this study, 34 paired samples of breast carcinomas and grossly nontumorous tissue from the same breast were cultured at 20 and 5% (12 samples) or 20 and 0% oxygen (22 samples). Both carcinoma samples and nontumorous tissue survived at 0% oxygen. Recovery for 24 hours at 20% produced good yields for cytogenetic analysis. Lower oxygen levels did not specifically stimulate growth of tumor cells. Samples with TP53 mutations showed a consistently increased growth under anaerobic hypoxic conditions. Culture at 5% oxygen did not generally reveal more karyotypic abnormalities than found at 20%. In the samples cultured at 0 and 20%, karyotypic abnormalities were detected only in anaerobic hypoxic culture in two cases. Of the only four samples where more complex karyotypes were detected in the low-oxygen culture, two were TP53 mutated. Hypoxic treatment followed by recovery at 20% oxygen may thus increase the yield of complex karyotypes from a...Continue Reading

References

Jul 1, 1992·Genes, Chromosomes & Cancer·N PandisF Mitelman
Jul 15, 1995·International Journal of Radiation Oncology, Biology, Physics·D M BrizelM W Dewhirst
Aug 1, 1997·Cytometry·M SteinardóttirH M Ogmundsdóttir
Jan 25, 2002·Genes, Chromosomes & Cancer·Kristine KleiviManuel R Teixeira
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Annika JögiSven Påhlman
Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Muhammad Al-HajjMichael F Clarke
May 3, 2003·Cancer Cell·Selma PennacchiettiPaolo M Comoglio
Dec 12, 2003·Nature Reviews. Cancer·Matthew Smalley, Alan Ashworth
Jan 23, 2004·Nature Reviews. Cancer·Ricardo PardalSean J Morrison
May 18, 2004·Trends in Genetics : TIG·Pascale BertrandBernard S Lopez
Nov 26, 2004·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Alexandra GiatromanolakiMichael I Koukourakis
May 4, 2005·Biochemical and Biophysical Research Communications·Anette Duensing, Stefan Duensing
May 4, 2005·Biochemical and Biophysical Research Communications·Ester M Hammond, Amato J Giaccia
May 4, 2005·Biochemical and Biophysical Research Communications·Steven E Artandi, Laura D Attardi

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Citations

Sep 13, 2011·Genes, Chromosomes & Cancer·Margret SteinarsdottirHelga M Ogmundsdottir

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