Effect of hypoxia on integrin-mediated adhesion of endothelial progenitor cells.

Journal of Cellular and Molecular Medicine
Ralf KaiserErik B Friedrich

Abstract

Homing of endothelial progenitor cells (EPCs) is crucial for neoangiogenesis, which might be negatively affected by hypoxia. We investigated the influence of hypoxia on fibronectin binding integrins for migration and cell-matrix-adhesion. AMP-activated kinase (AMPK) and integrin-linked kinase (ILK) were examined as possible effectors of hypoxia. Human EPCs were expanded on fibronectin (FN) and integrin expression was profiled by flow cytometry. Cell-matrix-adhesion- and migration-assays on FN were performed to examine the influence of hypoxia and AMPK-activation. Regulation of AMPK and ILK was shown by Western blot analysis. We demonstrate the presence of integrin β(1), β(2) and α(5) on EPCs. Adhesion to FN is reduced by blocking β(1) and α(5) (49% and 2% of control, P < 0.05) whereas α(4)-blockade has no effect. Corresponding effects were shown for migration. Hypoxia and AMPK-activation decrease adhesion on FN. Although total AMPK-expression remains unchanged, phospho-AMPK increases eightfold. The EPCs require α(5) for adhesion on FN. Hypoxia and AMPK-activation decrease adhesion. As α(5) is the major adhesive factor for EPCs on FN, this suggests a link between AMPK and α(5)-integrins. We found novel evidence for a connection ...Continue Reading

References

May 11, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·William A Muller
Jan 21, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Takako AkitaTsutomu Imaizumi
Jul 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Tianqing KongC Simon Shelley
Sep 2, 2004·Molecular and Cellular Biology·Erik B FriedrichRobert E Gerszten
Oct 19, 2004·Journal of the American College of Cardiology·Volker SchächingerAndreas M Zeiher
Dec 30, 2004·The Journal of Experimental Medicine·Emmanouil ChavakisStefanie Dimmeler
Sep 23, 2006·The New England Journal of Medicine·Birgit AssmusAndreas M Zeiher
Dec 1, 2006·Seminars in Cell & Developmental Biology·Elizabeth D Wederell, Robb U de Iongh
Apr 20, 2007·Human Pathology·Mayumi OkamuraYoshiaki Ishigatsubo
Feb 29, 2008·Journal of Molecular and Cellular Cardiology·Emmanouil ChavakisStefanie Dimmeler
Mar 4, 2008·Trends in Cell Biology·Tyisha Williams, Jay E Brenman
Sep 18, 2008·International Journal of Obesity : Journal of the International Association for the Study of Obesity·D G Hardie
Feb 28, 2009·Circulation Research·Eberhard SchulzThomas Münzel
Mar 10, 2009·Free Radical Biology & Medicine·Brooke M EmerlingNavdeep S Chandel
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Gregory R SteinbergMark A Febbraio
May 2, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Hou-Yu ChiangJane Sottile
Jul 3, 2009·American Journal of Physiology. Cell Physiology·Beth Ann MurphyVanessa H Routh

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Citations

Dec 5, 2013·Journal of Receptor and Signal Transduction Research·Lei XiaoQian Liu
Oct 30, 2016·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Kristy SwiderskiGordon Stuart Lynch
Jan 21, 2017·International Journal of Molecular Sciences·Rehman Ata, Costin N Antonescu
Jan 29, 2020·Cell Biochemistry and Function·Jyotirmaya BeheraSuvro Chatterjee
Oct 4, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Cansu YıldırımAnton J G Horrevoets

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Methods Mentioned

BETA
density gradient centrifugation
flow cytometry
fluorescence microscopy
FACS
flowcytometry

Software Mentioned

ScionPro
GraphPad
Excel
Prism

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