PMID: 7091491Feb 1, 1982Paper

Effect of immunomodulating factors present in ascitic fluids and sera from cancer patients on the responses of cultured mononuclear cells from normal subjects

American Journal of Reproductive Immunology : AJRI
J A Stratton, P J DiSaia

Abstract

Ascitic fluids and sera from patients with malignant tumors were tested for their ability to modulate the mitogen-induced blastogenic responses of normal subjects' peripheral blood mononuclear cells in vitro. The addition of either ascitic fluid or serum to cultures of normal blood cells greatly enhanced the blastogenic response of cells to phytohemagglutinin P, and markedly depressed the responses to concanavalin A and succinyl-concanavalin A. The blastogenic response of the cells to pokeweed mitogen was unaffected by the addition of serum and depressed by the addition of ascitic fluid. Autologous normal serum also enhanced the response to phytohemagglutinin P but had no effect on the response to the other mitogens. These activities were concentration-dependent and heat-stable (56 degrees C, 60 min) and could be detected even if the ascitic fluid or serum was added as late as the second day of culture. Cells that had been preincubated with serum or ascitic fluid and washed well before culturing with the mitogens responded in the same manner as cells cultured in the presence of serum or ascitic fluid. The mitogen-induced blastogenic responses of mononuclear cells were not affected by the addition of autologous cells that had be...Continue Reading

References

Jul 1, 1977·Cancer·P PerlmannG R Pape
Feb 1, 1978·Obstetrics and Gynecology·K UedaT Sugawa
Jan 15, 1971·International Journal of Cancer. Journal International Du Cancer·I HellströmG A Warner
Dec 12, 1974·The New England Journal of Medicine·A H GlasgowJ A Mannick

Citations

Apr 1, 1982·American Journal of Reproductive Immunology : AJRI·J A Stratton, P J DiSaia

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In Vitro [Publication Type]

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