PMID: 2505622Aug 1, 1989Paper

Effect of immunotoxins against Trypanosoma cruzi

The American Journal of Tropical Medicine and Hygiene
J M Santana, A R Teixeira

Abstract

Immunotoxins were constructed with IgG antibodies against Trypanosoma cruzi surface antigens by hybridization with abrin (ITA) and ricin (ITR) A chains. The biological activity of the hybrid macromolecules was tested on the parasite forms. Motility of parasite forms was lost in vitro after incubation with ITR. In general, killing of the parasite with ITR was more efficient than with ITA. Inhibition of protein synthesis after incubation with either ITR or ITA, measured by 3H-leucine incorporation, confirmed the parasite immobilization experiments. The lethal effect was potentiated when the immunotoxins were used in the presence of 2.5 mM ammonium chloride. T. cruzi antibodies specific to cell surface antigens are excellent drug carriers that can be delivered to the target cell. However, ITR and ITA did not reduce parasitemia or increase survival of mice infected with T. cruzi.

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

Related Papers

Laboratory Investigation; a Journal of Technical Methods and Pathology
A R Teixeira, J M Santana
The Journal of Immunology : Official Journal of the American Association of Immunologists
J M DerocqF K Jansen
The Journal of Immunology : Official Journal of the American Association of Immunologists
R J FultonE S Vitetta
International Journal of Cancer. Journal International Du Cancer
P CasellasK E Hellström
© 2022 Meta ULC. All rights reserved