Effect of implantation on engineered skeletal muscle constructs.

Journal of Tissue Engineering and Regenerative Medicine
Michael L WilliamsLisa M Larkin

Abstract

The development of engineered skeletal muscle would provide a viable tissue for replacement and repair of muscle damaged by disease or injury. Our current tissue-engineering methods result in three-dimensional (3D) muscle constructs that generate tension but do not advance phenotypically beyond neonatal characteristics. To develop to an adult phenotype, innervation and vascularization of the construct must occur. In this study, 3D muscle constructs were implanted into the hindlimb of a rat, along the sciatic nerve, with the sural nerve isolated, transected and sutured to the construct to encourage innervation. Aortic ring anchors were sutured to the tendons of the biceps femoris muscle so that the construct would move dynamically with the endogenous muscle. After 1 week in vivo, the constructs were explanted, evaluated for force production and stained for muscle, nerve and collagen markers. Implanted muscle constructs showed a developing capillary system, an epimysium-like outer layer of connective tissue and an increase in myofibre content. The beginning of α-bungarotoxin clustering suggests that neuromuscular junctions (NMJs) could form on the implanted muscle, given more time in vivo. Additionally, the constructs increased m...Continue Reading

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Citations

Dec 20, 2013·Regenerative Medicine·Jacob P MertensLisa M Larkin
Apr 24, 2014·Medical Hypotheses·Cameron J WilsonDevakara R Epari
Jan 23, 2016·Tissue Engineering. Part a·Brian C SyverudLisa Marie Larkin
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