Abstract
Rats were injected once iv with an islet-activating protein (IAP), a new protein purified from the culture medium of Bordetella pertussis. Three days later, their pancreases were studied in vitro for insulin secretory responses. As with pertussis vaccine, pretreatment of rats with IAP was effective in enhancing insulin release from pancreas during perfusion or from islets during incubation in response to secretagogues such as glucose and glibenclamide. The alpha-adrenergic inhibition of insulin secretion induced by epinephrine was also reversed by the pretreatment with IAP. 3-Isobutyl-1-methylxanthine caused insulin release due to accumulation of cAMP. This 3-isobutyl-1-methylxanthine-induced insulin release during perfusion was enhanced in a Ca-containing perfusate, but was conversely reduced in a Ca-free perfusate by the IAP pretreatment. Upon the addition of Ca to the Ca-free perfusate, more insulin was released from pancreases of IAP-treated rats than from those of nontreated rats.
Citations
Mar 1, 1983·Archives of Biochemistry and Biophysics·T MurayamaM Ui
Sep 30, 1985·Biochemical and Biophysical Research Communications·J D Veldhuis, E L Hewlett
Dec 15, 1981·Biochemical Pharmacology·M YajimaI Takahashi
Sep 28, 1984·Biochimica Et Biophysica Acta·K NogimoriM Ui
Sep 28, 1984·Biochimica Et Biophysica Acta·K NogimoriM Ui
Nov 22, 2000·Biochimie·G G HolzJ F Habener
Apr 12, 2012·American Journal of Physiology. Cell Physiology·Susanne G Straub, Geoffrey W G Sharp
Dec 5, 2012·Biological & Pharmaceutical Bulletin·Toshiaki Katada
Jul 21, 2010·The Journal of Physiology·Ying ZhaoGeoffrey W G Sharp
Aug 12, 2009·Molecular Aspects of Medicine·Fiorella Tonello, Cesare Montecucco
Aug 26, 2006·The European Journal of Neuroscience·Gerly A C BritoRonaldo A Ribeiro
Jan 1, 1982·Microbiology and Immunology·M Endoh, Y Nakase
Nov 15, 1983·Biochemical Pharmacology·T Nakamura, M Ui
Oct 26, 1982·Biochemistry·M TamuraS Ishii
Sep 11, 2014·American Journal of Physiology. Endocrinology and Metabolism·Veronika LeissBernd Nürnberg
Dec 1, 1996·The American Journal of Physiology·G W Sharp
Jan 30, 1984·Biochemical and Biophysical Research Communications·C Bernofsky, D G Amamoo