Effect of increased testicular temperature on seminal plasma proteome of the ram

Theriogenology
David R RochaA A Moura

Abstract

The present study evaluated the effects of heat stress on the ram seminal plasma proteome. Six Morada Nova rams were scrotal insulated for 8 days. Scrotal circumference, sperm parameters, and seminal fluid proteins were evaluated before (Day 0) and twice during scrotal insulation (Days 4 and 8), and weekly until semen parameters returned to preinsulation values (normal). Seminal proteins were analyzed by two-dimensional SDS-PAGE and mass spectrometry. Scrotal circumference decreased from 30 ± 0.4 cm on Day 0 to 22.6 ± 0.6 cm on Day 36 (P < 0.05) and became equivalent to preinsulation values on Day 71. Motile sperm became nearly absent from Day 8 to Day 64 but returned to normal on Day 113. Percentage of normal sperm changed similarly and returned to normal on Day 106. Rams were azoospermic between Days 29 and 64, and sperm concentration came back to normal on Day 92. The number of spots/two-dimensional gel reduced from 256 ± 31 on Day 0 to 104 ± 14 on Day 29 (when rams were azoospermic) and then increased to 183 ± 9 on Day 113 (P < 0.05), similar to spot counts before insulation. The intensities of 24 spots, referring to 17 seminal plasma proteins, were affected by treatment (P < 0.05). After insulation, seminal plasma had grea...Continue Reading

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Citations

Sep 17, 2016·Journal of Proteome Research·Taylor PiniSimon P de Graaf
Feb 23, 2020·Molecular Reproduction and Development·Gry Brandt Boe-HansenMichael McGowan
Oct 19, 2019·Reproduction in Domestic Animals = Zuchthygiene·Mercedes AlvarezLuis Anel
May 23, 2020·Theriogenology·Jane M Morrell
Jan 25, 2020·Theriogenology·Gabriel Ribas PereiraIvan Cunha Bustamante-Filho
Feb 26, 2021·Journal of Thermal Biology·Arnaldo Algaranhar GonçalvesOtávio Mitio Ohashi
Dec 12, 2018·Theriogenology·R K KasimanickamJ P Kastelic
Feb 18, 2019·Theriogenology·Eduarda EscobarFrancielli Weber Santos Cibin
Jun 6, 2021·Molecular & Cellular Proteomics : MCP·David A Skerrett-ByrneJohn E Schjenken
Oct 8, 2018·Theriogenology·Ana Beatriz Bossois MouraAlexandre Rossetto Garcia
Sep 24, 2021·Cell Stress & Chaperones·Shiva P SinghManmohan S Chauhan
Oct 10, 2021·BMC Genomics·David A Skerrett-ByrneJohn E Schjenken

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