Effect of increasing the expression of cholesterol transporters (StAR, MLN64, and SCP-2) on bile acid synthesis.

Journal of Lipid Research
S RenW M Pandak

Abstract

There are two major pathways of bile acid synthesis: the "neutral" pathway, initiated by highly regulated microsomal cholesterol 7alpha-hydroxylase (CYP7A1), and an "alternative" pathway, initiated by mitochondrial sterol 27-hydroxylase (CYP27A1). In hepatocyte cultures, overexpression of CYP7A1 increases bile acid synthesis by >8-fold. However, overexpression of CYP27A1 in hepatocytes only increases it by 1.5-fold, suggesting that additional rate-limiting steps must be involved in the regulation of this pathway. The effects of intracellular cholesterol transport proteins on bile acid synthesis have been investigated in the current study. Under culture conditions in which the neutral pathway was inactive, selective overexpression of the gene encoding steroidogenic acute regulatory protein (StAR), MLN64 (StAR homolog protein), and sterol carrier protein-2 (SCP-2) led to 5.7-, 1.2-, and 1.7-fold increases, respectively, in the rates of bile acid synthesis in primary rat hepatocytes. Surprisingly, co-overexpression of MLN64 with StAR, SCP-2, or CYP7A1 blunted the upregulated bile acid synthesis by 48, 47, and 45%, respectively. These results suggest that MLN64, in its full-length form, is not responsible for the transport of chole...Continue Reading

References

Apr 10, 1997·International Journal of Cancer. Journal International Du Cancer·C Moog-LutzM C Rio
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·H WatariJ F Strauss
Jul 31, 1998·Reviews of Reproduction·D M Stocco
Aug 5, 1999·Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [and] German Diabetes Association·D M Stocco
Mar 22, 2000·Chemistry and Physics of Lipids·A M GallegosF Schroeder
Jun 17, 2000·Biochimica Et Biophysica Acta·D M Stocco
Dec 9, 2000·Biochimica Et Biophysica Acta·L K Christenson, J F Strauss
Jun 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·E HallW Pandak
Sep 15, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·W M PandakZ R Vlahcevic
Oct 30, 2001·Experimental Biology and Medicine·F SchroederA B Kier
May 25, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·William M PandakZ Rano Vlahcevic
Jun 19, 2002·The Journal of Biological Chemistry·Mei ZhangJerome F Strauss
May 22, 2003·Metabolism: Clinical and Experimental·Shunlin RenWilliam M Pandak

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Citations

Sep 30, 2008·Reviews in Endocrine & Metabolic Disorders·Walter L Miller
Dec 1, 1978·The British Journal of Addiction to Alcohol and Other Drugs·V Berridge
Sep 1, 1977·Journal of Advanced Nursing·C Davies
Apr 11, 2008·Journal of Gastroenterology and Hepatology·Anna FernándezJosé C Fernandez-Checa
Jul 1, 1976·Journal of Neurochemistry·O P Sharma, C R Krishna Murti
Jan 1, 1991·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·M AltiniT Johnston
Aug 1, 1993·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·J H HellsteinK C Carriere
May 1, 1965·Acta paediatrica Scandinavica·P KARLBERGT K OLIVER
Jan 26, 2012·American Journal of Physiology. Endocrinology and Metabolism·Leyuan XuShunlin Ren
Oct 16, 2008·American Journal of Physiology. Endocrinology and Metabolism·Yongjie MaShunlin Ren
Oct 10, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Gregory G MartinFriedhelm Schroeder
Jun 9, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xin ZhangShunlin Ren
Apr 25, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Katie T BurkeLaura A Woollett
Jun 1, 2010·Journal of Lipid Research·Attilio RigottiSilvana Zanlungo
Apr 12, 2008·Journal of Lipid Research·Daniel Rodriguez-AgudoWilliam M Pandak
Oct 4, 2011·The Journal of Endocrinology·Barbara J Clark
Nov 27, 2014·Journal of Bioenergetics and Biomembranes·Laura A MartinBarbara Karten
Jan 19, 2016·Advanced Drug Delivery Reviews·Ru WenShanta Dhar
Jul 13, 2007·Biochemical and Biophysical Research Communications·Shunlin RenWilliam M Pandak
Nov 7, 2015·Archives of Biochemistry and Biophysics·Gregory G MartinFriedhelm Schroeder
Dec 14, 2005·Alcoholism, Clinical and Experimental Research·José C Fernandez-ChecaCarmen García-Ruiz
May 18, 2005·Journal of Lipid Research·Daniel Rodriguez-AgudoWilliam M Pandak
Mar 15, 2006·Journal of Lipid Research·Daniel Rodriguez-AgudoWilliam M Pandak
Jan 21, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lingpeng ZhuTing-Ting Wei
Sep 24, 2016·Clinical Science·Ugo Soffientini, Annette Graham
Oct 16, 2007·Molecular and Cellular Biochemistry·Songul Suren CastilloRefiye Yanardag

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