Effect of incubation temperature on the self-assembly of regenerated silk fibroin: a study using AFM

International Journal of Biological Macromolecules
Jian ZhongDannong He

Abstract

Understanding effect of temperature on the molecular self-assembly process will be helpful to unravel the structure-function relationship of biomolecule and to provide important information for the bottom-up approach to nanotechnology. In this work, the effect of incubation temperature on the secondary structures and morphological structures of regenerated silk fibroin (RSF) was systematically studied using atomic force microscopy and Fourier Transform infrared spectroscopy. The effect of incubation temperature on RSF self-assembly was dependent on RSF concentration. For the RSF solution with relatively low concentrations (15 μg/mL and 60 μg/mL), the increase of the incubation temperature mainly accelerated the formation and aggregation of antiparallel β-sheet protofibrils and decreased the formation of random coil protofilaments/globule-like molecules. For the RSF solution with relatively high concentrations (300 μg/mL and 1.5mg/mL), the increase of the incubation temperature mainly accelerated the formation and aggregation of antiparallel β-sheet RSF features (protofibrils and globule-like features) and decreased the formation of random coil bead-like features. This work implies that the morphology and conformation of biomacr...Continue Reading

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Citations

Oct 1, 2016·ACS Applied Materials & Interfaces·Banani KunduHae-Won Kim
Oct 14, 2016·Biomacromolecules·Benjamin P PartlowDavid L Kaplan
Jun 3, 2017·Biomacromolecules·Liying XiaoDavid L Kaplan
Jun 13, 2017·Biomacromolecules·Hyo Won KwakKi Hoon Lee
Apr 4, 2017·BioMed Research International·Hiep Thi NguyenToi Van Vo
Oct 6, 2020·Frontiers in Bioengineering and Biotechnology·Laise Maia LopesMarisa Masumi Beppu
Feb 12, 2021·International Journal of Biological Macromolecules·D V Santhosh Kumar GunapuSiva Rama Krishna Vanjari
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dakota PiorkowskiDeborah M Kane
Jan 18, 2018·ACS Sensors·Congzhou WangSrikanth Singamaneni
Sep 12, 2017·Biomacromolecules·Denis Mihaela PanaitescuRoxana Trusca

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