PMID: 8987102Jan 1, 1996Paper

Effect of indole-3-acetic acid (plant auxin) on the preservation at 15 degrees C of boar semen for artificial insemination

Reproduction, Nutrition, Development
R ToniolliY Combarnous

Abstract

In order to extend the duration of boar sperm survival at 15 degrees C for artificial insemination, we tested the effect of indole-3-acetic acid (IAA), which appeared to be the main sperm protective substance present in the Coco nucifera endosperm (coconut water). Two IAA concentrations (10 and 100 ng/mL) in Beltsville extender (BTS) were studied for their in vitro effects. The motility, the percentage of motile spermatozoa and the acrosome morphology of sperm were recorded each day over 13 days of storage at 15 degrees C, after 5 min and 3 h of incubation at 39 degrees C. The IAA effect on sperm preservation was also studied in vivo at a concentration of 10 ng/mL in BTS by inseminating groups of females twice at 24 h intervals either at D0 (day of sperm collection) and D1 (D0/1) or at D5 and D6 (D5/6). At D0/1, the two groups of females (control and IAA) were inseminated with a total of 6.3 x 10(9) spermatozoa (3.15 x 10(9) at D0 and the same dose at D1) whereas at D5/6, on IAA group was inseminated with a total of 6.3 x 10(9) spermatozoa and another one with 12.6 x 10(9) spermatozoa. The animals in the D5/6 control group were inseminated each with a total of 12.6 x 10(9) spermatozoa. For each group of females (n = 106-140), f...Continue Reading

Citations

Dec 29, 2010·Veterinary Medicine International·Evelyn Rabelo AndradeRicardo Toniolli
Dec 25, 2008·Cell Biochemistry and Function·Luciana R M B MourãoMariza P de Melo
Sep 18, 2018·Zygote : the Biology of Gametes and Early Embryos·José A B BezerraAlexandre R Silva
Jul 3, 2009·Reproduction in Domestic Animals = Zuchthygiene·A E F SilvaJ L Rodrigues
Oct 21, 2019·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Júlia Damé Fonseca PaschoalTiago Collares

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