Effect of Indoleamine 2,3-Dioxygenase Expressed in HTR-8/SVneo Cells on Decidual NK Cell Cytotoxicity

American Journal of Reproductive Immunology : AJRI
Yanli BanXun Qu

Abstract

To study the effect of indoleamine 2,3-dioxygenase (IDO) expressed in HTR-8/SVneo cells on NKG2D and NKp46 expression and cytotoxicity of decidual NK (dNK) and peripheral NK (pNK) cells. CD56(+) dNK and pNK cells purified were cultured with HTR-8/SVneo cell conditioned medium (CM), 1-MT+HTR-8/SVneo cell CM, and complete RPMI 1640 medium (negative control) in vitro. The mRNA and protein expression of NKG2D and NKp46 in NK cells were then assessed by qRT-PCR and flow cytometry, respectively. Their cytotoxicity was evaluated with LDH assays, and TNF-α secretion was analyzed by ELISA. For dNK cells, the mRNA and protein expression of NKp46 as well as NKG2D did not differ significantly among the three groups (P > 0.05), whereas for pNK cells, the expression level was significantly decreased in HTR-8/SVneo cell CM group than the other two groups (P < 0.01). Peripheral NK cells cultured with HTR-8/SVneo cell CM showed reduced cytotoxicity and TNF-α secretion than the other two groups (P < 0.01), although there were no significant differences among three groups for dNK cells (P > 0.05). IDO expressed by HTR-8/SVneo cells can down-regulate NKp46 and NKG2D expression and reduce cytotoxicity in pNK cells, and may contribute to keep dNK cy...Continue Reading

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Citations

Mar 3, 2017·American Journal of Reproductive Immunology : AJRI·Seo Y ParkSung K Lee
Jan 18, 2018·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Shao-Liang YangHai-Yan Wang
Mar 27, 2018·American Journal of Reproductive Immunology : AJRI·Maide OzenIrina Burd
May 31, 2019·Pharmacology Research & Perspectives·Yoshito NishimuraMasahiro Nishibori
May 3, 2018·Current Hypertension Reports·Layan Alrahmani, Maria Alice V Willrich
Jul 18, 2020·Cellular & Molecular Immunology·Xiao-Qiu Wang, Da-Jin Li
Dec 3, 2020·Cancers·Joanna DomagalaMagdalena Winiarska

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