Effect of inducible FHIT and p53 expression in the Calu-1 lung cancer cell line

Cancer Letters
A CavazzoniP G Petronini

Abstract

Loss of FHIT expression and p53 mutations are critical events in the early stages of lung carcinogenesis. The restoration of Fhit function in FHIT-negative cancer cells has been reported to cause tumour suppression by inhibition of cell proliferation and/or activation of apoptotic pathways. However, the studies designed to elucidate the biological role of Fhit and its potential interaction with p53 have produced conflicting results. We investigated here the effects of the simultaneous restoration of FHIT and p53 in Calu-1 cells by using a hormone-inducible gene expression system. We demonstrate that the restoration of FHIT expression reinforces the anti-proliferative effect associated with the simultaneous replacement of p53. Indeed, a more pronounced inhibition of cell proliferation associated with an earlier and higher induction of p21(waf1) mRNA and protein expression was observed in Fhit/p53-expressing cells compared with cells expressing p53 alone. This effect was not due to Fhit-mediated up-regulation of p53 expression; in fact p53 protein was expressed at the same level in both FHIT-positive and FHIT-negative cell clones. Consistent with this result, Fhit did not affect the expression of MDM2, a protein known to interact...Continue Reading

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Citations

Jan 30, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Po-Cheng ChiangJih-Hwa Guh
Sep 16, 2014·Advanced Biomedical Research·Ameneh EslamparastSoroush Sardari
Mar 20, 2012·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Francesca AndrianiLuca Roz
May 7, 2009·Journal of Cellular Physiology·Prisco MirandolaMarco Vitale
Apr 7, 2010·Biochemical Pharmacology·Maricla GalettiPier Giorgio Petronini
Sep 25, 2012·Cellular Signalling·Fereshteh Mir MohammadrezaeiMohammad Hossein Ghahremani
Jan 8, 2019·Journal of Cellular Biochemistry·Azam KhedriMohammad Hossein Ghahremani
Feb 19, 2008·Molecular Cancer Therapeutics·Andrea CavazzoniPier Giorgio Petronini

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