Effect of Inhibition of DNA Methylation Combined with Task-Specific Training on Chronic Stroke Recovery

International Journal of Molecular Sciences
In-Ae ChoiJongmin Lee

Abstract

To develop new rehabilitation therapies for chronic stroke, this study examined the effectiveness of task-specific training (TST) and TST combined with DNA methyltransferase inhibitor in chronic stroke recovery. Eight weeks after photothrombotic stroke, 5-Aza-2'-deoxycytidine (5-Aza-dC) infusion was done on the contralesional cortex for four weeks, with and without TST. Functional recovery was assessed using the staircase test, the cylinder test, and the modified neurological severity score (mNSS). Axonal plasticity and expression of brain-derived neurotrophic factor (BDNF) were determined in the contralateral motor cortex. TST and TST combined with 5-Aza-dC significantly improved the skilled reaching ability in the staircase test and ameliorated mNSS scores and cylinder test performance. TST and TST with 5-Aza-dC significantly increased the crossing fibers from the contralesional red nucleus, reticular formation in medullar oblongata, and dorsolateral spinal cord. Mature BDNF was significantly upregulated by TST and TST combined with 5-Azd-dC. Functional recovery after chronic stroke may involve axonal plasticity and increased mature BDNF by modulating DNA methylation in the contralesional cortex. Our results suggest that comb...Continue Reading

References

Dec 1, 1993·Stroke; a Journal of Cerebral Circulation·K Kogure, H Kato
Apr 18, 1998·Journal of Neurophysiology·J ClassenL G Cohen
Feb 5, 2000·Stroke; a Journal of Cerebral Circulation·H S JorgensenT S Olsen
Oct 25, 2000·British Journal of Neurosurgery·M C PapadopoulosB A Bell
Jan 22, 2001·The Journal of Biological Chemistry·S J MowlaR A Murphy
Oct 11, 2001·Neurobiology of Disease·S T CarmichaelT A Woolsey
Jul 9, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jung-Kil LeeStephen M Strittmatter
Aug 13, 2005·Experimental Brain Research·Steven C Cramer, Kit R Crafton
Dec 24, 2005·Archives of Physical Medicine and Rehabilitation·Sarah A MauldenGerben DeJong
Feb 13, 2008·Journal of Cellular and Molecular Medicine·A-M BugaA Popa-Wagner
Jan 24, 2009·Stroke; a Journal of Cerebral Circulation·Michelle PloughmanDale Corbett
Jun 9, 2009·Occupational Therapy International·Isobel J HubbardLeeanne M Carey
Nov 6, 2009·Nature Reviews. Neuroscience·Timothy H Murphy, Dale Corbett
Nov 26, 2009·Neurobiology of Disease·Larry I Benowitz, S Thomas Carmichael
May 7, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ke-Jie YinY Eugene Chen
Mar 11, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Andrew N ClarksonS Thomas Carmichael
Mar 16, 2011·Archives of Neurology·Irfan A Qureshi, Mark F Mehler
May 17, 2011·Lancet·Peter LanghorneGert Kwakkel
Jun 22, 2011·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Bruce Ovbiagele, Mai N Nguyen-Huynh
Jun 28, 2011·Neurorehabilitation and Neural Repair·Crystal L MacLellanDale Corbett
Apr 27, 2012·Stroke; a Journal of Cerebral Circulation·Jullet HanMarion S Buckwalter
Jul 12, 2012·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Lisa D MooreGuoping Fan
Jul 17, 2012·Neuroscience Letters·Min KongYing Wang
Jun 13, 2013·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Sophie SchweizerStefanie Märschenz
Oct 17, 2014·Expert Review of Neurotherapeutics·Antonio BerrettaAndrew N Clarkson
Feb 20, 2016·Molecular Neurobiology·Zhiping HuLiuwang Zeng
May 14, 2016·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Ya-Chao WangDirk M Hermann
Jun 23, 2016·Aging and Disease·Haiping ZhaoYumin Luo
Oct 21, 2016·BMB Reports·Dong-Hee ChoiJongmin Lee
Jun 9, 2017·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yu-Hui LinDong-Ya Zhu
Oct 7, 2017·Journal of the American Heart Association·Ying TangDong-Ya Zhu

❮ Previous
Next ❯

Citations

Aug 7, 2019·Journal of Neuroscience Research·Eric Zhang, Ping Liao
Jan 1, 2020·International Journal of Molecular Sciences·In-Ae ChoiJongmin Lee
Jan 17, 2020·Aging Cell·Daniel ChenDorota Skowronska-Krawczyk
Dec 21, 2018·International Journal of Molecular Sciences·Joen-Rong Sheu
Sep 2, 2020·International Journal of Molecular Sciences·Xianshuang LiuZhenggang Zhang
Dec 16, 2019·Neurochemistry International·Mario J BertogliatRaghu Vemuganti
Feb 22, 2019··Azadeh Yazdan-ShahmoradJia Wen Chan

❮ Previous
Next ❯

Software Mentioned

GOM Player
Axio Vision
SPSS
Windows
Image J

Related Concepts

Related Feeds

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.